Department of Prosthodontics, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China.
J Biomed Mater Res B Appl Biomater. 2018 Jan;106(1):410-420. doi: 10.1002/jbm.b.33843. Epub 2017 Feb 10.
This study evaluated biological compatibility and antibacterial activity of a bone substitute material consisting of silver-doped hydroxyapatite (AgHA) and Bio-Oss with different mixture ratios in vitro and investigated its antibacterial mechanism. AgHA was synthesized by a chemical precipitation method. After characterization, AgHA was mixed with Bio-Oss at three ratios: 1:1, 1:2, and 1:4 by weight. Then, Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn) were used to test the antibacterial activity of the mixture. Human periodontal ligament fibroblasts and rat bone marrow stromal cells were selected for cytocompatibility experiments. According to results, the peak value of the size of the AgHA was concentrated in the 100-200 nm range, and AgHA particles consisted of short rods. It was confirmed that the structure of AgHA was similar to that of standard hydroxyapatite. All three mixture ratios exhibited obvious antimicrobial properties, which increased with increasing AgHA. According to the effects on the expression of bacterial virulence genes, groups 1:1 and 1:2 both negatively affected Pg and Fn more significantly than group 1:4. Cytotoxicity experiments showed that 1:1 caused little cytotoxicity, while groups 1:2 and 1:4 exerted no significant cytotoxicity. Considering its biological compatibility and antibacterial activity, group 1:2 is the most recommended. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 410-420, 2018.
本研究通过体外实验评估了由载银羟基磷灰石（AgHA）和 Bio-Oss 组成的不同混合比例的骨替代材料的生物相容性和抗菌活性，并研究了其抗菌机制。AgHA 通过化学沉淀法合成。经过表征后，将 AgHA 与 Bio-Oss 以 1:1、1:2 和 1:4 的重量比混合。然后，使用牙龈卟啉单胞菌（Pg）和核梭杆菌（Fn）测试混合物的抗菌活性。选择人牙周膜成纤维细胞和大鼠骨髓基质细胞进行细胞相容性实验。结果表明，AgHA 粒径的峰值集中在 100-200nm 范围内，AgHA 颗粒由短棒组成。证实了 AgHA 的结构与标准羟基磷灰石相似。所有三种混合比例均表现出明显的抗菌性能，且随着 AgHA 的增加而增加。根据对细菌毒力基因表达的影响，1:1 和 1:2 组对 Pg 和 Fn 的影响均明显大于 1:4 组。细胞毒性实验表明，1:1 组几乎没有细胞毒性，而 1:2 组和 1:4 组则没有明显的细胞毒性。考虑到其生物相容性和抗菌活性，1:2 组是最推荐的。©2017Wiley Periodicals, Inc. J 生物材料研究杂志 B：应用生物材料，106B：410-420，2018。
