Biomaterials Research Group, Bioengineering Program, Engineering Faculty, University of Antioquia, Medellin, Colombia.
Department of Chemical Engineering, Northeastern University, Boston, Massachusetts.
J Biomed Mater Res A. 2018 Nov;106(11):2984-2993. doi: 10.1002/jbm.a.36488. Epub 2018 Oct 26.
A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth. Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses. The potential for developing an injectable bone substitute (IBS) was investigated here. In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated. Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells). MTS viability testing indicated that osteoblast adhesion and viability on the IBS were excellent and uniform among various formulation types. Bacteria assays were also performed to assess antimicrobial functions on the CG/nHA composite against both Gram-negative and Gram-positive strains. A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS. In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2984-2993, 2018.
一个成功的术后植入物与加速的恢复周期有关，涉及到有效再生丢失或非存活组织，并减少微生物的生长。或者，植入物的长期成功取决于选择一种工程仿生材料，该材料具有生物相容性、不可生物降解性和在植入部位的稳定性，而不会引起任何非必要或不良的生物学反应。本研究探讨了可注射骨替代物（IBS）的开发潜力。特别是，通过化学交联制备了卡拉胶（CG）和纳米羟基磷灰石（nHA）可注射复合材料，并评估了哺乳动物细胞和细菌在 IBS 表面结构上的体外行为。然后，通过重量计评估了由 1%、1.5%和 2.5% CG 和 60% nHA 组成的配方与人类成骨细胞（或骨形成细胞）的相互作用。MTS 生存力测试表明，IBS 上的成骨细胞粘附和活力在各种配方类型之间均良好且均匀。还进行了细菌测定，以评估 CG/nHA 复合材料对革兰氏阴性和革兰氏阳性菌株的抗菌功能。发现一些样本中的 CG 含量较高与铜绿假单胞菌生长抑制的改善相关，尽管其他细菌菌株不受 IBS 影响。总之，这项研究强调了 CG/nHA 复合材料作为创新生物材料，应进一步研究以降低细菌活性并促进成骨细胞反应，而无需使用药物。© 2018 威利父子公司。J 生物医学材料研究部分 A：106A：2984-2993，2018 年。
