Dekio S, Hashimoto K, Makino M
Department of Dermatology and Syphilology, Wayne State University School of Medicine, Detroit, Michigan.
Zentralbl Bakteriol. 1989 Oct;271(4):442-51. doi: 10.1016/s0934-8840(89)80103-3.
Ribosomal proteins (r-proteins) from a type I strain (ATCC 11827) and a type II strain (ATCC 11828) of Propionibacterium acnes (P. acnes) and from a Propionibacterium granulosum (P. granulosum) strain (ATCC 11829-2) were analyzed using two-dimensional polyacrylamide gel electrophoresis (two-dimensional PAGE), for the purpose of genetical analysis of the P. acnes type I and type II strains and the P. granulosum strain. Among the fifty r-proteins in all, almost all of the proteins were electrophoretically different between both of the two types of P. acnes strains and the P. granulosum strain, whereas only five proteins were different between the type I and type II strains of P. acnes. Such differences of r-protein composition between the type I and type II strains of P. acnes indicate that the type I strain is genetically different from, but very closely related to, the type II strain. However, the P. granulosum strain is not so closely related to both the two types of P. acnes strains. This study thus suggests that the two-dimensional PAGE of r-proteins is an excellent tool for the taxonomy of P. acnes and the related bacteria.
为了对痤疮丙酸杆菌（P. acnes）I型菌株（ATCC 11827）、II型菌株（ATCC 11828）以及颗粒丙酸杆菌（P. granulosum）菌株（ATCC 11829-2）进行遗传学分析，使用二维聚丙烯酰胺凝胶电泳（二维PAGE）对来自这些菌株的核糖体蛋白（r蛋白）进行了分析。在总共50种r蛋白中，两种痤疮丙酸杆菌菌株与颗粒丙酸杆菌菌株之间的几乎所有蛋白质在电泳上都存在差异，而痤疮丙酸杆菌I型和II型菌株之间只有5种蛋白质不同。痤疮丙酸杆菌I型和II型菌株之间r蛋白组成的这种差异表明，I型菌株在遗传上与II型菌株不同，但关系非常密切。然而，颗粒丙酸杆菌菌株与两种痤疮丙酸杆菌菌株的关系并非如此密切。因此，这项研究表明，r蛋白的二维PAGE是痤疮丙酸杆菌及相关细菌分类学的一种优秀工具。
