Department of Medical Biosciences/Pathology, Umeå University, Sweden.
Anaerobe. 2013 Oct;23:48-54. doi: 10.1016/j.anaerobe.2013.07.002. Epub 2013 Jul 27.
Propionibacterium acnes (P. acnes) and Propionibacterium granulosum (P. granulosum) are common skin colonizers that are implicated as possible contributing factors in acne vulgaris development. We have established direct visualization tools for the simultaneous detection of these closely related species with immunofluorescence assay and fluorescence in situ hybridization (FISH). As proof of principle, we were able to distinguish P. acnes and P. granulosum bacteria in multi-species populations in vitro as well as in a mock skin infection model upon labelling with 16S rRNA probes in combinatorial FISH as well as with antibodies. Furthermore, we report the co-localization of P. acnes and P. granulosum in the stratum corneum and hair follicles from patients with acne vulgaris as well as in healthy individuals. Further studies on the spatial distribution of these bacteria in skin structures in various skin disorders are needed.
痤疮丙酸杆菌（P. acnes）和颗粒丙酸杆菌（P. granulosum）是常见的皮肤定植菌，它们被认为是寻常痤疮发展的可能致病因素。我们已经建立了直接可视化工具，用于通过免疫荧光法和荧光原位杂交（FISH）同时检测这些密切相关的物种。作为原理验证，我们能够在体外多物种群体以及模拟皮肤感染模型中，通过组合 FISH 以及抗体标记 16S rRNA 探针来区分 P. acnes 和 P. granulosum 细菌。此外，我们报告了寻常痤疮患者和健康个体的角质层和毛囊中 P. acnes 和 P. granulosum 的共定位。需要进一步研究这些细菌在各种皮肤疾病的皮肤结构中的空间分布。
