Montánchez Itxaso, Kaberdina Anna Chao, Sevillano Elena, Gallego Lucía, Rodríguez-Couto Susana, Kaberdin Vladimir R
Department of Immunology, Microbiology and Parasitology, University of the Basque Country UPV/EHU, Leioa, Spain.
Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU), P.O. Box 644, 48080, Bilbao, Spain.
Folia Microbiol (Praha). 2017 Jul;62(4):325-334. doi: 10.1007/s12223-017-0501-3. Epub 2017 Feb 10.
Polychlorinated aromatic compounds, including pentachlorobenzenes and hexachlorobenzenes, are recalcitrant industrial pollutants that cause adverse effects on living cells. In this paper, the isolation of Pseudomonas fluorescens species with high resistance to pentachlorobenzene (PeCB) is reported. It was found that, in contrast to its slightly negative effect on P. fluorescens growth, PeCB readily inhibited the cell growth of Serratia spp. and Escherichia coli strains, thus indicating that inhibition of bacterial growth by PeCB is species-dependent. Analysis of a P. fluorescens isolate revealed that the exposure to PeCB induced production of reactive oxygen species and led to an increase in the level of alkyl hydroperoxide reductase C (AhpC), an important enzyme enhancing the cell tolerance to organic hydroperoxides usually accumulated under oxidative stress. The putative mechanism conferring PeCB resistance to P. fluorescens and the potential use of P. fluorescens in bioremediation are discussed.
多氯芳香族化合物，包括五氯苯和六氯苯，是顽固的工业污染物，会对活细胞产生不利影响。本文报道了对五氯苯（PeCB）具有高抗性的荧光假单胞菌的分离。研究发现，与PeCB对荧光假单胞菌生长的轻微负面影响相反，PeCB很容易抑制沙雷氏菌属和大肠杆菌菌株的细胞生长，这表明PeCB对细菌生长的抑制具有物种依赖性。对一株荧光假单胞菌的分析表明，暴露于PeCB会诱导活性氧的产生，并导致烷基过氧化氢还原酶C（AhpC）水平升高，AhpC是一种重要的酶，可增强细胞对通常在氧化应激下积累的有机过氧化氢的耐受性。文中讨论了赋予荧光假单胞菌对PeCB抗性的推定机制以及荧光假单胞菌在生物修复中的潜在用途。
