使用 TripleTOF 质谱仪为无标记定量蛋白质组学研究生成高质量的 SWATH 采集数据。
Generation of High-Quality SWATH Acquisition Data for Label-free Quantitative Proteomics Studies Using TripleTOF Mass Spectrometers.
作者信息
Schilling Birgit, Gibson Bradford W, Hunter Christie L
机构信息
The Buck Institute for Research on Aging, 1201 Radio Road, Redwood City, 94065, CA, USA.
SCIEX, 500 Old Connecticut Path, Framingham, MA, 01701, USA.
出版信息
Methods Mol Biol. 2017;1550:223-233. doi: 10.1007/978-1-4939-6747-6_16.
Abstract
Data-independent acquisition is a powerful mass spectrometry technique that enables comprehensive MS and MS/MS analysis of all detectable species, providing an information rich data file that can be mined deeply. Here, we describe how to acquire high-quality SWATH Acquisition data to be used for large quantitative proteomic studies. We specifically focus on using variable sized Q1 windows for acquisition of MS/MS data for generating higher specificity quantitative data.
摘要
数据非依赖型采集是一种强大的质谱技术，能够对所有可检测物种进行全面的质谱和串联质谱分析，提供一个可深度挖掘的信息丰富的数据文件。在此，我们描述如何获取高质量的SWATH采集数据，用于大规模定量蛋白质组学研究。我们特别着重于使用可变大小的Q1窗口来采集串联质谱数据，以生成更高特异性的定量数据。
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