Ogata Sho, Shimizu Ken, Tominaga Susumu, Nakanishi Kuniaki
Department of Laboratory Medicine, National Defense Medical College Hospital, Tokorozawa, Saitama 359-8513, Japan; Department of Diagnostic Pathology, JCHO Saitama Medical Center, Saitama, Saitama 330-0074, Japan.
Department of Diagnostic Pathology, JCHO Saitama Medical Center, Saitama, Saitama 330-0074, Japan.
Hum Pathol. 2017 Apr;62:126-133. doi: 10.1016/j.humpath.2017.01.013. Epub 2017 Feb 8.
Most patients with human intestinal spirochetosis (HIS; a colorectal bacterial infection caused by Brachyspira species) seem asymptomatic, and its pathogenicity remains unclear. Recently, alterations in mucin expression were reported in animal Brachyspira infection. The present question was "Is mucin expression altered in HIS?" Using antibodies for MUCs 1, 2, 4, 5AC, and 6, we immunohistochemically compared 215 specimens from 83 histology-confirmed HIS cases with 106 specimens from 26 non-HIS cases. Positive staining (which included even focal positive staining) was rated "high (+)" or "low (+)." Results were analyzed for 4 categories of lesions, and associations between MUC expression and spirochetal presence were also analyzed. In the "specimens without polyps or adenocarcinoma" category, high (+) MUC2 positivity was more frequent in HIS than in control. In the hyperplasia/serrated polyp category, in HIS (versus control), the MUC5AC positivity rate was lower, whereas high (+) MUC4 positivity was more frequent. In the conventional adenoma category, in HIS (versus control), the MUC1 positivity rate was lower, whereas both high (+) MUC2 positivity and high (+) MUC5AC positivity were less frequent. In the adenocarcinoma category, high (+) MUC2 positivity was more frequent in HIS than in control. Among the above mucins, only MUC1 positivity was significantly associated with an absence of the so-called fringe formation, an absence of spiral organisms within mucus, and an absence of strong immunopositive materials within the epithelial layer and within the subepithelial layer. The results suggest that Brachyspira infection or a related change in the microbiome may alter the large intestine mucin expression profile in humans.
大多数人肠道螺旋体病（HIS，一种由短螺旋体属细菌引起的结肠直肠细菌感染）患者似乎没有症状，其致病性仍不清楚。最近，有报道称动物感染短螺旋体后粘蛋白表达发生改变。目前的问题是“HIS中粘蛋白表达是否改变？”我们使用针对MUC1、MUC2、MUC4、MUC5AC和MUC6的抗体，对83例经组织学确诊的HIS病例的215份标本与26例非HIS病例的106份标本进行了免疫组织化学比较。阳性染色（包括即使是局灶性阳性染色）被评为“高（+）”或“低（+）”。对4类病变的结果进行了分析，还分析了粘蛋白表达与螺旋体存在之间的关联。在“无息肉或腺癌的标本”类别中，HIS中MUC2高（+）阳性比对照组更常见。在增生性/锯齿状息肉类别中，与对照组相比，HIS中MUC5AC阳性率较低，而MUC4高（+）阳性更常见。在传统腺瘤类别中，与对照组相比，HIS中MUC1阳性率较低，而MUC2高（+）阳性和MUC5AC高（+）阳性均较少见。在腺癌类别中，HIS中MUC2高（+）阳性比对照组更常见。在上述粘蛋白中，只有MUC1阳性与所谓的边缘形成缺失、粘液内无螺旋体生物以及上皮层和上皮下层内无强免疫阳性物质显著相关。结果表明，短螺旋体感染或微生物群的相关变化可能会改变人类大肠粘蛋白的表达谱。
