Alkarmi T, Alshakarchi Z, Behbehani K, Ali-Khan Z
Department of Microbiology, Faculty of Medicine, University of Kuwait, Safat.
Br J Exp Pathol. 1989 Oct;70(5):579-88.
Alveolar hydatid cysts, the larvae of Echinococcus multilocularis, were shown to induce the formation of an amyloid enhancing-like factor (AEF) called alveolar hydatid cyst-AEF (AHC-AEF) in the spleens of infected animals, 5 to 6 weeks post-infection. The adoptive transfer of AHC-AEF results in rapid induction and deposition of amyloid fibrils in the spleens following AgNO3 injection within 24 h. When injected concomitantly with alveolar hydatid cysts, it shortens the induction phase from 5 or 12 weeks to 1 week in C57BL/6J and A/JAX mice respectively. Preliminary characterization of this factor indicates that its bioactivity may be detected in the non-lipid moiety of spleen cell extracts and the sediment of ultracentrifugation. The factor is resistant to trypsin, pepsin, DNA-ase and RNA-ase treatments. It is sensitive to low or high pH (pH 3.0 and 9.5) and boiling. It resists freeze drying, dialysis, and storage at 4 degrees C for 2 weeks or -70 degrees C for more than a year. It may be fractionated using borate buffered saline, pH 7.4, on Sephacryl S-300 gel column. The active moiety eluted mainly in the first peak.
泡型包虫囊肿是多房棘球绦虫的幼虫，在感染动物感染后5至6周，其可在感染动物脾脏中诱导形成一种名为泡型包虫囊肿淀粉样增强因子（AHC-AEF）的淀粉样增强因子样因子。AHC-AEF的过继转移导致在注射硝酸银后24小时内脾脏中迅速诱导并沉积淀粉样纤维。当与泡型包虫囊肿同时注射时，在C57BL/6J和A/JAX小鼠中，它分别将诱导期从5周或12周缩短至1周。该因子的初步表征表明，其生物活性可在脾细胞提取物的非脂质部分和超速离心沉淀物中检测到。该因子对胰蛋白酶、胃蛋白酶、DNA酶和RNA酶处理具有抗性。它对低pH或高pH（pH 3.0和9.5）及煮沸敏感。它能耐受冻干、透析以及在4℃下储存2周或在-70℃下储存一年以上。它可用pH 7.4的硼酸盐缓冲盐水在Sephacryl S-300凝胶柱上进行分级分离。活性部分主要在第一个峰中洗脱出来。
