Abankwa G V, Ali-Khan Z
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.
Br J Exp Pathol. 1988 Feb;69(1):123-32.
The biological activity and time of appearance of alveolar hydatid cyst induced splenic amyloid enhancing factor (AEF) with respect to amyloid deposition in the spleens were determined in C57BL/6J mice. Mice were infected intraperitoneally with 100 alveolar hydatid cysts (AHC) and killed bi-weekly between 2 and 14 weeks postinfection (p.i.). AHCs and spleens were excised, weighed and a portion of each spleen was sectioned and stained for quantitation of amyloid deposits and histological studies. The remaining spleen pieces were sonicated separately in cold phosphate buffered saline, pH 7.4 (I g/10 ml), centrifuged (27,000 g, 60 min, 4 degrees C) and the supernatant tested for AEF activity. Splenomegaly followed the progressive increase in the AHC biomass and AEF activity coincided with the appearance of amyloid deposits at 6 weeks p.i. A 2.5 mg intraperitoneal protein dosage of AEF in conjunction with a subcutaneous injection of 0.5 ml of a 0.11 M AgNO3 solution in mice, induced the maximum amount of splenic amyloid deposition at 48 h; the amount of splenic amyloid deposits decreased by either increasing or decreasing the AEF dosage. In vivo, 70% of the AEF activity was abolished by day 4 post-injection of AEF and completely by 3 weeks. These findings indicate that AHC-induced AEF is functionally analogous to casein-induced AEF and its appearance in the spleen coincides with neutrophilia, histiocytosis and amyloid deposition.
在C57BL/6J小鼠中，确定了肺泡包虫囊肿诱导的脾淀粉样增强因子（AEF）的生物活性及其在脾脏中淀粉样沉积出现的时间。小鼠经腹腔注射100个肺泡包虫囊肿（AHC），在感染后（p.i.）2至14周期间每两周处死一批。切除AHC和脾脏，称重，将每个脾脏的一部分进行切片并染色，用于淀粉样沉积的定量分析和组织学研究。将剩余的脾脏组织分别在冰冷的pH 7.4磷酸盐缓冲盐溶液（1 g/10 ml）中超声处理，离心（27,000 g，60分钟，4℃），并检测上清液中的AEF活性。脾肿大随着AHC生物量的逐渐增加而出现，AEF活性与感染后6周淀粉样沉积的出现相一致。在小鼠腹腔内注射2.5 mg AEF蛋白，并皮下注射0.5 ml 0.11 M硝酸银溶液，在48小时时诱导出最大量的脾淀粉样沉积；增加或减少AEF剂量，脾淀粉样沉积量均会减少。在体内，注射AEF后第4天，70%的AEF活性被消除，3周时完全消除。这些发现表明，AHC诱导的AEF在功能上类似于酪蛋白诱导的AEF，其在脾脏中的出现与嗜中性粒细胞增多、组织细胞增多和淀粉样沉积同时发生。
