Alkarmi T O, Alshakarchi Z, Behbehani K
Department of Microbiology, Faculty of Medicine, Kuwait University.
Parasite Immunol. 1988 Jul;10(4):443-57. doi: 10.1111/j.1365-3024.1988.tb00234.x.
Alveolar hydatid cysts (AHC) were isolated from C57BL/6J and BALB/c mice at 8 and 12 weeks post-infection from subcutaneous and intraperitoneal foci and cultured in vitro. Freshly isolated as well as in vitro-grown cysts were incubated with Fc or F(ab')2 fragments of human, rabbit, mouse, goat or sheep immunoglobulins, then washed and incubated with a fluorescein-conjugated rabbit or goat F(ab')2 fraction of antisera to each of the above primary sera. Significant fluorescence on the surface of AHC was detected when they were incubated with human, rabbit or mouse Fc fractions followed by the addition of goat or rabbit fluorescein-conjugated antiserum. No fluorescence was detected when the AHC were incubated with the F(ab')2 fragments and fluorescein-conjugated antiserum, except when the primary antiserum was mouse F(ab')2 fractions. Cysts grown in vitro retained their binding ability to human, rabbit or mouse Fc fractions. However, the intensity of fluorescence decreased proportionally with time. The maximum intensity of staining was observed with small cysts. Large cysts showed diminished fluorescence. The non-specific binding of human, mouse or rabbit immunoglobulins (Fc fractions) to AHC was also confirmed by rosetting with sheep erythrocytes (SRBC) which suggests that the larvae of Echinococcus multilocularis may utilize the presence of Fc receptors on their surface in order to elude immune destruction by the host.
在感染后8周和12周，从C57BL/6J和BALB/c小鼠的皮下和腹腔病灶中分离出肺泡包虫囊肿（AHC），并进行体外培养。将新鲜分离的以及体外培养的囊肿与人类、兔子、小鼠、山羊或绵羊免疫球蛋白的Fc或F(ab')2片段孵育，然后洗涤，并与荧光素偶联的兔或山羊F(ab')2抗血清孵育，该抗血清针对上述每种主要血清。当AHC与人、兔子或小鼠的Fc片段孵育，随后加入山羊或兔子荧光素偶联抗血清时，在其表面检测到显著荧光。当AHC与F(ab')2片段和荧光素偶联抗血清孵育时，未检测到荧光，除非主要抗血清是小鼠F(ab')2片段。体外培养的囊肿保留了它们与人、兔子或小鼠Fc片段的结合能力。然而，荧光强度随时间成比例下降。在小囊肿中观察到最大染色强度。大囊肿显示荧光减弱。人、小鼠或兔子免疫球蛋白（Fc片段）与AHC的非特异性结合也通过与绵羊红细胞（SRBC）的玫瑰花结形成得到证实，这表明多房棘球绦虫幼虫可能利用其表面Fc受体的存在来逃避宿主的免疫破坏。
