Jeong Sook-Hyang, Park Byung-Joo, Kim Yong-Hyun, Choi Yun Suk, Ahn Hee-Seop, Han Sang-Hoon, Choi In-Soo
Department of Internal Medicine, Seoul National University Bundang Hospital, College of Medicine, Seoul National University, Seongnam, Gyeonggi 13620, Republic of Korea.
Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
J Clin Virol. 2017 Apr;89:10-13. doi: 10.1016/j.jcv.2017.02.002. Epub 2017 Feb 6.
Autochthonous hepatitis E occurs sporadically in developed countries. The consumption of undercooked pork containing hepatitis E virus genotype 3 (HEV-3) or 4 (HEV-4) is the major risk factor for infection. The serological diagnostic kits currently used in hospitals sometimes produce false-negative or -positive results. Therefore, detection of both HEV RNA and antibodies to the virus is required for confirmative diagnosis of hepatitis E.
We aimed to detect HEV in serum samples from patients with cryptogenic hepatitis and to determine the origin of HEV.
A nested polymerase chain reaction (PCR) method was developed for detection of HEV-3 and HEV-4 in patients with hepatitis. A total of 23 serum samples, deposited in 2006-2012, from patients with acute cryptogenic hepatitis who were serologically negative for hepatitis A, B, C, and E were examined using this method. The amplified PCR products were genetically analyzed.
Four HEV-4 isolates were detected from the 23 serum samples. Phylogenetic analysis indicated that three of the four isolates were closely related to HEV-4 isolates found in pigs in Korea and in patients with hepatitis E in Japan.
The newly developed nested PCR method was useful for detection of HEV in patients with cryptogenic hepatitis. The close relationship between the human HEV-4 isolates identified in this study and swine isolates implied that zoonotic transmission of HEV might be a source of infection in patients with hepatitis.
在发达国家，散发性戊型肝炎呈零星发生。食用含有戊型肝炎病毒3型（HEV-3）或4型（HEV-4）的未煮熟猪肉是感染的主要危险因素。目前医院使用的血清学诊断试剂盒有时会产生假阴性或假阳性结果。因此，戊型肝炎的确证诊断需要同时检测HEV RNA和病毒抗体。
我们旨在检测不明原因肝炎患者血清样本中的HEV，并确定HEV的来源。
开发了一种巢式聚合酶链反应（PCR）方法，用于检测肝炎患者中的HEV-3和HEV-4。使用该方法对2006年至2012年期间收集的23份血清样本进行检测，这些样本来自甲型、乙型、丙型和戊型肝炎血清学检测均为阴性的急性不明原因肝炎患者。对扩增的PCR产物进行基因分析。
从23份血清样本中检测到4株HEV-4分离株。系统发育分析表明，4株分离株中的3株与在韩国猪和日本戊型肝炎患者中发现的HEV-4分离株密切相关。
新开发的巢式PCR方法可用于检测不明原因肝炎患者中的HEV。本研究中鉴定的人类HEV-4分离株与猪分离株之间的密切关系表明，HEV的人畜共患病传播可能是肝炎患者的感染源。
Zotero 插件