采用大脑中动脉闭塞技术诱导小鼠缺血性中风和缺血再灌注以及梗死区域可视化
Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area.
作者信息
Bertrand Luc, Dygert Levi, Toborek Michal
机构信息
Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami; Miller School of Medicine, University of Miami.
Miller School of Medicine, University of Miami.
出版信息
J Vis Exp. 2017 Feb 2(120):54805. doi: 10.3791/54805.
Abstract
Cerebrovascular disease is highly prevalent in the global population and encompasses several types of conditions, including stroke. To study the impact of stroke on tissue injury and to evaluate the effectiveness of therapeutic interventions, several experimental models in a variety of species were developed. They include complete global cerebral ischemia, incomplete global ischemia, focal cerebral ischemia, and multifocal cerebral ischemia. The model described in this protocol is based on the middle cerebral artery occlusion (MCAO) and is related to the focal ischemia category. This technique produces consistent focal ischemia in a strictly defined region of the hemisphere and is less invasive than other methods. The procedure described is performed on mice, given the availability of several genetic variants and the high number of tests standardized for mice to aid in the behavioral and neurodeficit evaluation.
摘要
脑血管疾病在全球人口中高度流行，涵盖多种病症，包括中风。为了研究中风对组织损伤的影响并评估治疗干预措施的有效性，人们开发了多种物种的几种实验模型。它们包括完全性全脑缺血、不完全性全脑缺血、局灶性脑缺血和多灶性脑缺血。本方案中描述的模型基于大脑中动脉闭塞（MCAO），属于局灶性缺血类别。该技术在半球的严格定义区域产生一致的局灶性缺血，并且比其他方法侵入性更小。鉴于有多种基因变体以及针对小鼠的大量标准化测试可用于行为和神经功能缺损评估，所描述的程序是在小鼠身上进行的。
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