Cheng Yi, Zhang Jinjing, Deng Liancheng, Johnson Noah R, Yu Xichong, Zhang Ning, Lou Tianzheng, Zhang Yi, Wei Xiaojie, Chen Zaifeng, He Songbin, Li Xiaokun, Xiao Jian
Institute of Molecular Pharmacology, School of Pharmaceutics Science, Wenzhou Medical University Wenzhou 325035, PR China ; Present address: Department of Pharmacy, Longyou People's Hospital Quzhou, Zhejiang, 324400, PR China.
Institute of Molecular Pharmacology, School of Pharmaceutics Science, Wenzhou Medical University Wenzhou 325035, PR China.
Int J Clin Exp Pathol. 2015 Mar 1;8(3):2928-36. eCollection 2015.
Stem cell transplantation may provide an alternative therapy to promote functional recovery after various neurological disorders including cerebral infarct. Due to the minimal immunogenicity and neuronal differentiation potential of neural stem cells (NSCs), we tested whether intravenous administration of mice-derived C17.2 NSCs could improve neurological function deficit and cerebral infarction volume after ischemic stroke in rats. Additionally, we evaluated the survival, migration, proliferation, and differentiation capacity of transplanted NSCs in the rat brain. Intravenous infusion of NSCs after middle cerebral artery occlusion (MCAO) showed better performance in neurobiological severity scores after MCAO compared to control. However, the volume of cerebral infarction was not different at 7 days after MCAO compared with control. Transplanted NSCs were detected in the ischemic region but not in the contralateral hemisphere. NSCs differentiated into neurons or astrocytes after MCAO. These data suggest that intravenously transplanted NSCs can migrate, proliferate, and differentiate into neurons and astrocytes in the rat brain with focal ischemia and improve functional recovery.
干细胞移植可能为促进包括脑梗死在内的各种神经系统疾病后的功能恢复提供一种替代疗法。由于神经干细胞（NSCs）的免疫原性极小且具有神经元分化潜能，我们测试了静脉注射小鼠来源的C17.2神经干细胞是否能改善大鼠缺血性中风后的神经功能缺损和脑梗死体积。此外，我们评估了移植的神经干细胞在大鼠脑中的存活、迁移、增殖和分化能力。大脑中动脉闭塞（MCAO）后静脉输注神经干细胞与对照组相比，在MCAO后的神经生物学严重程度评分中表现更好。然而，与对照组相比，MCAO后7天的脑梗死体积没有差异。在缺血区域检测到移植的神经干细胞，但在对侧半球未检测到。MCAO后神经干细胞分化为神经元或星形胶质细胞。这些数据表明，静脉移植的神经干细胞可以在大鼠局灶性缺血脑中迁移、增殖并分化为神经元和星形胶质细胞，并改善功能恢复。
