UR 1268 Biopolymères Interactions Assemblages, INRA , F-44300 Nantes, France.
UP 2012.10.101 EGEAL Unit, Unilasalle , F-60000 Beauvais, France.
J Agric Food Chem. 2017 Aug 9;65(31):6442-6451. doi: 10.1021/acs.jafc.6b05371. Epub 2017 Mar 1.
Wheat allergy is an IgE-mediated disorder. Polyphenols, which are known to interact with certain proteins, could be used to reduce allergic reactions. This study screened several polyphenol sources for their ability to interact with gliadins, mask epitopes, and affect basophil degranulation. Polyphenol extracts from artichoke leaves, cranberries, apples, and green tea leaves were examined. Of these extracts, the first three formed insoluble complexes with gliadins. Only the cranberry and apple extracts masked epitopes in dot blot assays using anti-gliadin IgG and IgE antibodies from patients with wheat allergies. The cranberry and artichoke extracts limited cellular degranulation by reducing mouse anti-gliadin IgE recognition. In conclusion, the cranberry extract is the most effective polyphenol source at reducing the immunogenicity and allergenicity of wheat gliadins.
小麦过敏是一种 IgE 介导的疾病。多酚已知可与某些蛋白质相互作用，可用于减少过敏反应。本研究筛选了几种多酚来源，以研究其与麦醇溶蛋白相互作用、掩盖表位和影响嗜碱性粒细胞脱颗粒的能力。研究了朝鲜蓟叶、蔓越莓、苹果和绿茶叶中的多酚提取物。在这些提取物中，前三种与麦醇溶蛋白形成不溶性复合物。只有蔓越莓和苹果提取物在使用来自小麦过敏患者的抗麦醇溶蛋白 IgG 和 IgE 抗体的斑点印迹分析中掩盖了表位。蔓越莓和朝鲜蓟提取物通过减少小鼠抗麦醇溶蛋白 IgE 的识别来限制细胞脱颗粒。总之，蔓越莓提取物是降低小麦醇溶蛋白免疫原性和变应原性最有效的多酚来源。
