Yang Tao, Zhu Fang, Zhou Tianxiao, Cao Jianzhong, Xie Yibo, Zhang Meiyi, Wang Yan, Cao Dong-Sheng, Lin Qinlu, Zhang Lin
National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology.
Anal Sci. 2017;33(2):191-196. doi: 10.2116/analsci.33.191.
This study aimed to develop a label-free, sensitive, selective, and environment-friendly fluorescent peptide probe His-His-Trp-His (HHWH) for determining the concentration of copper ion (Cu) in aqueous solutions. The results demonstrated that the designed HHWH has a high selectivity and sensitivity for monitoring the concentration of free Cu via quenching of the probe fluorescence upon a binding of Cu. The fluorescence intensity of the HHWH had a linear relationship with the concentration of Cu between 10 nM and 10 μM, and the detection limit was 8 nM. Furthermore, HHWH could be regenerated with sulfide ions at least five times. The concentrations of Cu in three different real water samples were detected using this probe, and the results were consistent with the one detected using an atomic absorption spectrometer. Thus, HHWH can be used as an accurate and feasible fluorescent peptide probe for detecting Cu in aqueous solutions.
本研究旨在开发一种无标记、灵敏、选择性好且环境友好的荧光肽探针His-His-Trp-His（HHWH），用于测定水溶液中铜离子（Cu）的浓度。结果表明，所设计的HHWH对通过Cu结合时探针荧光猝灭来监测游离Cu的浓度具有高选择性和灵敏度。HHWH的荧光强度与10 nM至10 μM之间的Cu浓度呈线性关系，检测限为8 nM。此外，HHWH可用硫化物离子再生至少五次。使用该探针检测了三种不同实际水样中的Cu浓度，结果与使用原子吸收光谱仪检测的结果一致。因此，HHWH可作为一种准确可行的荧光肽探针用于检测水溶液中的Cu。
