Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin 150030, PR China.
Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin 150030, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Feb 5;208:198-205. doi: 10.1016/j.saa.2018.10.005. Epub 2018 Oct 8.
A highly sensitive fluorescent probe, 2‑butyl‑6‑(2‑ethylidenehydrazinyl)‑1H‑benzo[de]isoquinoline‑1,3(2H)‑dione (P) has been designed and synthesized to detect Cu in CHCN-HEPES (4:1, v/v, pH = 7.4) solution. This probe functions via a distinctive hydrolysis mechanism through Cu-promoted accompany by extinction of the fluorescent and other competing metal ions did not showed any interference. The limit of detection toward Cu was calculated of 320 nM. Probe P was not only successfully used for the determination of trace level Cu in the CHCN-HEPES (4:1, v/v, pH = 7.4) solution, but also valid for fluorescence imaging of Cu in lysosomes of 293T cells and it was applied in real water samples. This work indicated that P would be of great application prospect in environmental monitoring and medical diagnosis.
一种高灵敏度的荧光探针，2-丁基-6-（2-亚乙基肼基）-1H-苯并[de]异喹啉-1,3(2H)-二酮（P）已被设计和合成，用于在 CHCN-HEPES（4:1，v/v，pH=7.4）溶液中检测 Cu。该探针通过独特的水解机制起作用，通过 Cu 促进伴随荧光的消光，而其他竞争金属离子没有显示出任何干扰。对 Cu 的检测限计算为 320 nM。探针 P 不仅成功地用于测定 CHCN-HEPES（4:1，v/v，pH=7.4）溶液中的痕量 Cu，而且还可以用于 293T 细胞溶酶体中 Cu 的荧光成像，并且已应用于实际水样。这项工作表明，P 在环境监测和医学诊断方面具有广阔的应用前景。
