Ishihara Ryo, Uchino Yoshitaka, Hosokawa Kazuo, Maeda Mizuo, Kikuchi Akihiko
Department of Materials Science and Technology, Tokyo University of Science.
Anal Sci. 2017;33(2):197-202. doi: 10.2116/analsci.33.197.
We propose an easy microchannel surface functionalization method for a poly(dimethylsiloxane) (PDMS) microchip that utilizes electron beam-induced graft polymerization (EIGP) as a platform for microchip-based biomarker analysis. Unlike other grafting techniques, EIGP enables rapid surface modification of PDMS without initiator immobilization. The grafted microchip is preservable, and can be easily functionalized for versatile applications. In this study, the surface-functionalized power-free microchip (SF-PF microchip) was used for the detection of microRNA (miRNA), which is a biomarker for many serious diseases. The EIGP enables high-density three-dimensional probe DNA immobilization, resulting in rapid and sensitive miRNA detection on the portable SF-PF microchip. The limit of detection was 0.8 pM, the required sample volume was 0.5 μL, and the analysis time was 15 min. The SF-PF microchip will be a versatile platform for microchip-based point-of-care diagnosis.
我们提出了一种用于聚二甲基硅氧烷（PDMS）微芯片的简易微通道表面功能化方法，该方法利用电子束诱导接枝聚合（EIGP）作为基于微芯片的生物标志物分析平台。与其他接枝技术不同，EIGP能够在不固定引发剂的情况下对PDMS进行快速表面改性。接枝后的微芯片可保存，并且可以很容易地进行功能化以用于多种应用。在本研究中，表面功能化无电源微芯片（SF-PF微芯片）用于检测微小RNA（miRNA），miRNA是许多严重疾病的生物标志物。EIGP能够实现高密度三维探针DNA固定，从而在便携式SF-PF微芯片上实现快速灵敏的miRNA检测。检测限为0.8 pM，所需样品体积为0.5 μL，分析时间为15分钟。SF-PF微芯片将成为基于微芯片的即时诊断的通用平台。
