Faculty of Medicine, Juntendo University, 1-1 Hirakagakuendai, Inzai, Chiba, 270-1695, Japan.
Department of Materials Science & Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo, 125-8585, Japan.
Anal Sci. 2021 May 10;37(5):747-751. doi: 10.2116/analsci.20SCP17. Epub 2021 Jan 22.
Circulating microRNAs (miRNAs) have emerged as promising cancer biomarkers because their concentration profiles in body fluids are associated with the type and clinical stage of cancer. For multiplex miRNA detection, a novel surface-functionalized power-free microfluidic chip (SF-PF microchip) has been developed. The inner surface of the SF-PF microchip microchannels was functionalized via electron beam-induced graft polymerization and immobilization of capture probe DNAs. Simultaneous and specific duplex miRNA detection was achieved on the line-type SF-PF microchip with detection limits of 19.1 and 47.6 nmol L for hsa-miR-16 and hsa-miR-500a-3p, respectively. Moreover, simultaneous and specific triplex miRNA detection was achieved on the stripe-type SF-PF microchip. The sample volume required for this microchip was 0.5 μL, and the time required for detection was 17 min. These results indicate that up to six types of miRNAs could be detected without compromising the advantages of the previous SF-PF microchips for cancer point-of-care testing.
循环 microRNAs（miRNAs）已成为有前途的癌症生物标志物，因为它们在体液中的浓度谱与癌症的类型和临床阶段有关。为了进行多重 miRNA 检测，开发了一种新型的表面功能化无电源微流控芯片（SF-PF 微芯片）。SF-PF 微芯片微通道的内表面通过电子束诱导接枝聚合和捕获探针 DNA 的固定化进行功能化。在线型 SF-PF 微芯片上实现了同时且特异性的双链 miRNA 检测，hsa-miR-16 和 hsa-miR-500a-3p 的检测限分别为 19.1 和 47.6 nmol L。此外，在条纹型 SF-PF 微芯片上实现了同时且特异性的三链 miRNA 检测。该微芯片所需的样本体积为 0.5 μL，检测所需的时间为 17 分钟。这些结果表明，在不影响以前用于癌症即时检测的 SF-PF 微芯片优势的情况下，多达 6 种类型的 miRNA 可以被检测到。
