Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province, School of Engineering, Sun Yat-sen University , Guangzhou 510275, China.
Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.
Anal Chem. 2017 Feb 21;89(4):2561-2568. doi: 10.1021/acs.analchem.6b04785. Epub 2017 Feb 2.
Ozone (O) would be harmful to human skin for its strong oxidizing property, especially when stratum corneum or corneal epithelium is wounded. Imaging the penetration and distribution of ozone at depth is beneficial for studying the influence of ozone on skin or eyes. Here, we introduced a facile method for three-dimensional (3D) imaging of the penetration of O into the anterior chamber of an isolated crucian carp eye by using optical coherence tomography (OCT) combined with gold triangular nanoprisms (GTNPs) as the contrast agent and molecular probe. We illustrated the specific response of GTNPs to ozone and demonstrated that GTNPs can function as an efficient nanoprobe for sensing O. The stabilities of GTNPs in different biologic solutions, as well as the signal intensity of GTNPs on an OCT imaging system, were investigated. Visualization of 3D penetration and distribution of O in the biologic tissue was proved for the first time. The quantitative analysis of O diffusion in the anterior chamber of the fish eye revealed a penetration depth of 311 μm within 172 min. Due to the strong scattering, near-infrared extinction band, and easy functionalization of GTNPs, they could further serve as nanoprobes for 3D OCT or multimodal imaging of other molecules or ions in the future.
臭氧（O）因其强氧化性对人体皮肤有害，尤其是当角质层或角膜上皮受损时。想象臭氧在深度上的渗透和分布有助于研究臭氧对皮肤或眼睛的影响。在这里，我们介绍了一种通过光学相干断层扫描（OCT）结合金三角纳米棱镜（GTNPs）作为对比剂和分子探针，对鲫鱼眼睛前房内 O 渗透进行三维（3D）成像的简便方法。我们说明了 GTNPs 对臭氧的特殊反应，并证明 GTNPs 可以作为一种有效的纳米探针来检测 O。研究了 GTNPs 在不同生物溶液中的稳定性以及 GTNPs 在 OCT 成像系统上的信号强度。首次证明了在生物组织中 O 渗透和分布的可视化。对鱼眼前房内 O 扩散的定量分析表明，在 172 分钟内渗透深度为 311μm。由于 GTNPs 具有强散射、近红外消光带和易于功能化的特点，它们将来还可以进一步用作纳米探针，用于其他分子或离子的 3D OCT 或多模态成像。
