利用飞行时间二次离子质谱技术快速单细胞模式化研究 HeLa 细胞药物诱导的表型改变。
Fast Single-Cell Patterning for Study of Drug-Induced Phenotypic Alterations of HeLa Cells Using Time-of-Flight Secondary Ion Mass Spectrometry.
机构信息
Department of Chemistry, ‡Division of Biomedical Engineering, §Materials Characterization and Preparation Facility, Department of Chemical and Biomolecular Engineering, and ∥Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology , Hong Kong, China.
出版信息
Anal Chem. 2016 Dec 20;88(24):12196-12203. doi: 10.1021/acs.analchem.6b03170. Epub 2016 Dec 2.
A facile single-cell patterning (ScP) method was developed and integrated with time-of-flight secondary ion mass spectrometry (TOF-SIMS) for the study of drug-induced cellular phenotypic alterations. Micropatterned poly(dimethylsiloxane) (PDMS) stencil film and centrifugation-assisted cell trapping were combined for the preparation of on-surface single-cell microarrays, which exhibited both high site occupancy (>90%) and single-cell resolution (>97%). TOF-SIMS is a surface-sensitive mass spectrometry and is increasingly utilized in biological studies. Here we demonstrated, for the first time, its successful application in high-throughput single-cell analysis. Drug-induced phenotypic alterations of HeLa cells in the early stage of apoptosis were investigated using TOF-SIMS. The major molecular sources of variations were analyzed by principle component analysis (PCA).
开发了一种简便的单细胞图案化（ScP）方法，并将其与飞行时间二次离子质谱（TOF-SIMS）集成，用于研究药物诱导的细胞表型改变。微图案化聚二甲基硅氧烷（PDMS）掩模薄膜和离心辅助细胞捕获相结合，用于制备表面上单细胞微阵列，其表现出高的位点占有率（>90%）和单细胞分辨率（>97%）。TOF-SIMS 是一种表面敏感的质谱分析技术，在生物学研究中越来越多地得到应用。在这里，我们首次证明了它在高通量单细胞分析中的成功应用。使用 TOF-SIMS 研究了 HeLa 细胞在早期凋亡阶段的药物诱导的表型改变。通过主成分分析（PCA）分析了变异的主要分子来源。
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