Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education and Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education and Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
Biosens Bioelectron. 2018 Mar 15;101:146-152. doi: 10.1016/j.bios.2017.10.027. Epub 2017 Oct 16.
A novel magnetic controlled photoelectrochemical (PEC) sensing system was designed for sensitive detection of prostate-specific antigen (PSA) using reduced graphene oxide-functionalized BiFeO (rGO-BiFeO) as the photoactive material and target-triggered hybridization chain reaction (HCR) for signal amplification. Remarkably enhanced PEC performance could be obtained by using rGO-BiFeO as the photoelectrode material due to its accelerated charge transfer and improved the visible light absorption. Additionally, efficient and simple operation could be achieved by introducing magnetic controlled flow-through device. The assay mainly involved in anchor DNA-conjugated magnetic bead (MB-aDNA), PSA aptamer/trigger DNA (Apt-tDNA) and two glucose oxidase-labeled hairpins (H1-GOx and H2-GOx). Upon addition of target PSA, the analyte initially reacted with the aptamer to release the trigger DNA, which partially hybridized with the anchor DNA on the MB. Thereafter, the unpaired trigger DNA on the MB opened the hairpin DNA structures in sequence and propagated a chain reaction of hybridization events between two alternating hairpins to form a long nicked double-helix with numerous GOx enzymes on it. Subsequently, the enzymatic product (HO) generated and consumed the photo-excited electrons from rGO-BiFeO under visible light irradiation to enhance the photocurrent. Under optimal conditions, the magnetic controlled PEC sensing system exhibited good photocurrent responses toward target PSA within the linear range of 0.001 - 100ng/mL with a detection limit of 0.31pg/mL. Moreover, favorable selectivity, good stability and satisfactory accuracy were obtained. The excellent analytical performance suggested that the rGO-BiFeO-based PEC sensing platform could be a promising tool for sensitive, efficient and low cost detection of PSA in disease diagnostics.
一种新型的磁控光电化学（PEC）传感系统被设计用于使用还原氧化石墨烯功能化的 BiFeO（rGO-BiFeO）作为光活性材料，通过目标触发的杂交链式反应（HCR）进行信号放大，以灵敏检测前列腺特异性抗原（PSA）。由于 rGO-BiFeO 加速了电荷转移并提高了可见光吸收，因此将其用作光电电极材料可获得显著增强的 PEC 性能。此外，通过引入磁控流通过装置可以实现高效且简单的操作。该测定主要涉及到与磁珠偶联的锚 DNA（MB-aDNA）、PSA 适体/触发 DNA（Apt-tDNA）和两个葡萄糖氧化酶标记的发夹（H1-GOx 和 H2-GOx）。加入靶标 PSA 后，分析物最初与适体反应以释放触发 DNA，触发 DNA 部分与 MB 上的锚 DNA 杂交。此后，MB 上未配对的触发 DNA 依次打开发夹 DNA 结构，并在两个交替发夹之间进行杂交事件的链式反应，形成带有许多 GOx 酶的长切口双链。随后，在可见光照射下，酶产物（HO）生成并消耗 rGO-BiFeO 的光激电子，从而增强光电流。在最佳条件下，磁控 PEC 传感系统对 0.001-100ng/mL 范围内的目标 PSA 表现出良好的光电流响应，检测限为 0.31pg/mL。此外，还获得了良好的选择性、稳定性和令人满意的准确性。优异的分析性能表明，基于 rGO-BiFeO 的 PEC 传感平台有望成为疾病诊断中用于灵敏、高效和低成本检测 PSA 的有前途的工具。
