Correia Patrícia A, Lottem Eran, Banerjee Dhruba, Machado Ana S, Carey Megan R, Mainen Zachary F
Champalimaud Neuroscience Program, Champalimaud Centre for the Unknown, Lisbon, Portugal.
School of Medicine, University of California, Irvine, United States.
Elife. 2017 Feb 14;6:e20975. doi: 10.7554/eLife.20975.
Serotonin (5-HT) is associated with mood and motivation but the function of endogenous 5-HT remains controversial. Here, we studied the impact of phasic optogenetic activation of 5-HT neurons in mice over time scales from seconds to weeks. We found that activating dorsal raphe nucleus (DRN) 5-HT neurons induced a strong suppression of spontaneous locomotor behavior in the open field with rapid kinetics (onset ≤1 s). Inhibition of locomotion was independent of measures of anxiety or motor impairment and could be overcome by strong motivational drive. Repetitive place-contingent pairing of activation caused neither place preference nor aversion. However, repeated 15 min daily stimulation caused a persistent increase in spontaneous locomotion to emerge over three weeks. These results show that 5-HT transients have strong and opposing short and long-term effects on motor behavior that appear to arise from effects on the underlying factors that motivate actions.
血清素（5-羟色胺，5-HT）与情绪和动机相关，但内源性5-HT的功能仍存在争议。在此，我们研究了在从秒到周的时间尺度上，对小鼠5-HT神经元进行相位光遗传学激活的影响。我们发现，激活中缝背核（DRN）的5-HT神经元会在旷场中以快速动力学（起效时间≤1秒）强烈抑制自发运动行为。运动抑制与焦虑或运动损伤的指标无关，并且可以被强烈的动机驱动所克服。激活的重复位置相关配对既不会导致位置偏好也不会导致厌恶。然而，每天重复15分钟的刺激会导致在三周内自发运动持续增加。这些结果表明，5-HT瞬变对运动行为具有强烈且相反的短期和长期影响，这似乎源于对激发行动的潜在因素的影响。
