Cinti Stefano, Basso Mattia, Moscone Danila, Arduini Fabiana
Department of Chemical Science and Technology, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133 Rome, Italy.
Department of Chemical Science and Technology, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133 Rome, Italy.
Anal Chim Acta. 2017 Apr 1;960:123-130. doi: 10.1016/j.aca.2017.01.010. Epub 2017 Jan 23.
Herein, we report the first example of a paper-based screen-printed biosensor for the detection of ethanol in beer samples. Common office paper was adopted to fabricate the analytical device. The properties of this paper-based screen-printed electrode (SPE) were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy, and they were compared with the well-established polyester-based SPEs as well. Paper demonstrated similar properties when compared with polyester, highlighting suitability towards its utilization in sensor development, with the advantages of low cost and simple disposal by incineration. A nanocomposite formed by Carbon Black (CB) and Prussian Blue nanoparticles (PBNPs), namely CB/PBNPs, was utilized as an electrocatalyst to detect the hydrogen peroxide generated by the enzymatic reaction between alcohol oxidase (AOx) and ethanol. After optimizing the analytical parameters, such as pH, enzyme, concentration, and working potential, the developed biosensor allowed a facile quantification of ethanol up to 10 mM (0.058 %), with a sensitivity of 9.13 μA/mM cm (1574 μA/% cm) and a detection limit equal to 0.52 mM (0.003%). These satisfactory performances rendered the realized paper-based biosensor reliable over the analysis of ethanol contained in four different types of beers, including Pilsner, Weiss, Lager, and alcohol-free. The proposed manufacturing approach offers an affordable and sustainable tool for food quality control and for the realization of different electrochemical sensors and biosensors as well.
在此，我们报道了首例用于检测啤酒样品中乙醇的纸质丝网印刷生物传感器。采用普通办公纸制作分析装置。通过循环伏安法、电化学阻抗谱和扫描电子显微镜对这种纸质丝网印刷电极（SPE）的性能进行了研究，并与成熟的聚酯基SPE进行了比较。与聚酯相比，纸张表现出相似的性能，突出了其在传感器开发中的适用性，具有低成本和通过焚烧简单处置的优点。由炭黑（CB）和普鲁士蓝纳米颗粒（PBNPs）形成的纳米复合材料，即CB/PBNPs，被用作电催化剂来检测醇氧化酶（AOx）与乙醇之间的酶促反应产生的过氧化氢。在优化了诸如pH值、酶、浓度和工作电位等分析参数后，所开发的生物传感器能够轻松定量高达10 mM（0.058%）的乙醇，灵敏度为9.13 μA/mM cm（1574 μA/% cm），检测限为0.52 mM（0.003%）。这些令人满意的性能使得所实现的纸质生物传感器在分析包括比尔森啤酒、小麦啤酒、贮藏啤酒和无醇啤酒在内的四种不同类型啤酒中的乙醇时具有可靠性。所提出的制造方法为食品质量控制以及实现不同的电化学传感器和生物传感器提供了一种经济且可持续的工具。
