Novak J L, Wheeler B C
Department of Electrical and Computer Engineering, University of Illinois Urbana-Champaign 61801.
Brain Res. 1989 Sep 18;497(2):223-30. doi: 10.1016/0006-8993(89)90266-7.
Recordings of epileptiform burst activity in the CA1 region of the transverse rat hippocampal slices were made with a 32-channel surface electrode array. The 200 microns interelectrode resolution, the simultaneity of the data, and the use of a two-dimensional current source density analysis allowed accurate measurement of population spike peak times. Differences were found in the apparent propagation delays among 3 burst components: the compound action potential (CAP) along the Schaffer collaterals, the first population spike directly driven by the CAP, and the second (and succeeding) population spikes representing the bursting, epileptiform component. Delay measurements were applied to epileptiform bursts recorded in slices treated with picrotoxin (PTX), pentylenetetrazol (PTZ), and 0-Mg2+ medium. In 0-Mg2+ medium all components propagate at nearly the same velocity. In the PTZ and PTX media the second population spike propagated more slowly than the CAP. The first population spike propagated at the same velocity as the CAP for orthodromic Schaffer collateral stimulation. The first population spike propagated at the same, slower velocity as the second spike for antidromic Schaffer collateral stimulation.
使用32通道表面电极阵列对横向大鼠海马切片CA1区的癫痫样爆发活动进行记录。电极间分辨率为200微米，数据具有同步性，且采用二维电流源密度分析，能够精确测量群体峰电位的峰值时间。在3个爆发成分的明显传播延迟方面发现了差异：沿Schaffer侧支的复合动作电位（CAP）、由CAP直接驱动的第一个群体峰电位以及代表爆发性癫痫样成分的第二个（及后续）群体峰电位。延迟测量应用于用荷包牡丹碱（PTX）、戊四氮（PTZ）和无镁（0-Mg2+）培养基处理的切片中记录的癫痫样爆发。在无镁培养基中，所有成分以几乎相同的速度传播。在PTZ和PTX培养基中，第二个群体峰电位的传播速度比CAP慢。对于正向Schaffer侧支刺激，第一个群体峰电位与CAP以相同速度传播。对于逆向Schaffer侧支刺激，第一个群体峰电位与第二个峰电位以相同的较慢速度传播。
