Sorbonne Universités-Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, CS 60319, 60203, Compiègne cedex, France.
Université Libanaise, Laboratoire de Valorisation des Ressources Naturelles et Produits de Santé, Cité Universitaire Rafic Hariri, Hadath, Lebanon.
Angew Chem Int Ed Engl. 2017 Mar 13;56(12):3339-3343. doi: 10.1002/anie.201612667. Epub 2017 Feb 14.
An enzyme-mediated synthetic approach is described for the preparation of molecularly imprinted polymer nanoparticles (MIP-NPs) in aqueous media. Horseradish peroxidase (HRP) was used to initiate the polymerization of methacrylate or vinyl monomers and cross-linkers by catalyzing the generation of free radicals. To prevent entrapment of the enzyme in the cross-linked polymer, and to enable it to be reused, HRP was immobilized on a solid support. MIPs based on 4-vinylpyridine and 1,4-bis(acryloyl)piperazine for the recognition of 2,4-dichlorophenoxyacetic acid (2,4-D) and salicylic acid were synthesized in an aqueous medium. MIPs for the protein trypsin were also synthesized. MIP nanoparticles with sizes between 50 and 300 nm were obtained with good binding properties, a good imprinting effect, and high selectivity for the target molecule. The reusability of immobilized HRP for MIP synthesis was shown for several batches.
本文描述了一种在水相介质中酶介导的合成方法，用于制备分子印迹聚合物纳米颗粒（MIP-NPs）。辣根过氧化物酶（HRP）通过催化自由基的生成来引发甲基丙烯酸酯或乙烯基单体和交联剂的聚合。为了防止酶被包埋在交联聚合物中，并使其能够重复使用，HRP 被固定在固体载体上。以 4-乙烯基吡啶和 1,4-双（丙烯酰基）哌嗪为模板分子，合成了用于识别 2,4-二氯苯氧乙酸（2,4-D）和水杨酸的 MIP。还合成了用于蛋白质胰蛋白酶的 MIP。在水相中得到了粒径在 50-300nm 之间的 MIP 纳米颗粒，具有良好的结合性能、良好的印迹效果和对目标分子的高选择性。固定化 HRP 用于 MIP 合成的可重复使用性在几批中得到了证明。
