Butelli Eugenio, Garcia-Lor Andrés, Licciardello Concetta, Las Casas Giuseppina, Hill Lionel, Recupero Giuseppe Reforgiato, Keremane Manjunath L, Ramadugu Chandrika, Krueger Robert, Xu Qiang, Deng Xiuxin, Fanciullino Anne-Laure, Froelicher Yann, Navarro Luis, Martin Cathie
John Innes Centre, Norwich NR4 7UH, United Kingdom (E.B., L.H., C.M.);
Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain (A.G.-L., L.N.);
Plant Physiol. 2017 Apr;173(4):2225-2242. doi: 10.1104/pp.16.01701. Epub 2017 Feb 14.
Mandarin (), citron (), and pummelo () are important species of the genus and parents of the interspecific hybrids that constitute the most familiar commercial varieties of : sweet orange, sour orange, clementine, lemon, lime, and grapefruit. Citron produces anthocyanins in its young leaves and flowers, as do species in genera closely related to , but mandarins do not, and pummelo varieties that produce anthocyanins have not been reported. We investigated the activity of the gene, which encodes a MYB transcription factor controlling anthocyanin biosynthesis, in different accessions of a range of species and in domesticated cultivars. A white mutant of lemon lacks functional alleles of , demonstrating that plays an essential role in anthocyanin production in Almost all the natural variation in pigmentation by anthocyanins in species can be explained by differences in activity of the gene, caused by point mutations and deletions and insertions of transposable elements. Comparison of the allelic constitution of in different species and cultivars also helps to clarify many of the taxonomic relationships in different species of , confirms the derivation of commercial varieties during domestication, elucidates the relationships within the subgenus , and allows a new genetic classification of mandarins.
宽皮橘、枸橼和柚是柑橘属的重要物种，也是构成柑橘最常见商业品种的种间杂种的亲本：甜橙、酸橙、克莱门氏小柑橘、柠檬、酸橙和葡萄柚。枸橼在其幼叶和花朵中产生花青素，与柑橘属密切相关的属中的物种也是如此，但宽皮橘不产生，并且尚未报道过产生花青素的柚品种。我们研究了一个编码控制花青素生物合成的MYB转录因子的基因在一系列柑橘属物种的不同种质以及驯化品种中的活性。柠檬的一个白色突变体缺乏该基因的功能等位基因，这表明该基因在柑橘属花青素产生中起关键作用。几乎所有柑橘属物种中花青素色素沉着的自然变异都可以由该基因活性的差异来解释，这些差异是由点突变、转座元件的缺失和插入引起的。比较不同物种和品种中该基因的等位基因组成也有助于阐明柑橘属不同物种之间的许多分类关系，确认驯化过程中商业品种的起源，阐明亚属内的关系，并允许对宽皮橘进行新的遗传分类。
