Research Institute of Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
Technological Innovation Team of Basic Theory Study Research of Institution of Higher Education in Heilongjiang Province, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
Chin J Integr Med. 2019 Sep;25(9):663-670. doi: 10.1007/s11655-016-2741-5. Epub 2017 Feb 15.
To investigate the mechanism of Chinese herbal medicine Dioscorea nipponica for the treatment of monosodium urate crystals-induced gouty arthritis (GA) in rats.
Sixty male Wistar rats were divided into 6 groups: normal, model, indomethacin and three total saponin (900, 300 and 100 mg/kg) groups. The liver, kidney and serum levels of lysosomal enzymes, antioxidant capacities, and inflammatory factors were measured. In addition, the mRNA and protein levels of the NALP3 inflammasome components in the mononuclear cells of rats' peripheral blood were analyzed using real-time polymerase chain reaction and Western blotting methods, respectively.
Total saponins groups could reduce the activities of β-galactosidase, β-N acetyl glucosamine enzyme, β-glucuronidase, acid phosphatase, and malonaldehyde as well as the contents of TNF-α, IL-1β and IL-8 (all P<0.05). They could also increase the activities of glutathione peroxidase and total superoxide dismutase (both P<0.05). Further studies showed that total saponins groups of high, middle and low doses could all increase the mRNA and protein levels of caspase-1, adapter apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) and NALP3 in the mononuclear cells of peripheral blood (all P<0.05).
Dioscorea nipponica may treat GA by regulating lysosomal enzymes, antioxidant capacities and the NALP3 inflammasome.
研究中药穿山龙治疗尿酸单钠晶体诱导的大鼠痛风性关节炎（GA）的作用机制。
将 60 只雄性 Wistar 大鼠分为 6 组：正常组、模型组、吲哚美辛组和穿山龙总皂苷 900、300、100mg/kg 组。测定肝、肾和血清中溶酶体酶、抗氧化能力和炎症因子的水平。此外，采用实时聚合酶链反应和 Western blot 法分别分析大鼠外周血单个核细胞中 NALP3 炎性体成分的 mRNA 和蛋白水平。
穿山龙总皂苷各剂量组可降低β-半乳糖苷酶、β-N 乙酰氨基葡萄糖酶、β-葡糖苷酸酶、酸性磷酸酶和丙二醛的活性以及 TNF-α、IL-1β和 IL-8 的含量（均 P<0.05），还可增加谷胱甘肽过氧化物酶和总超氧化物歧化酶的活性（均 P<0.05）。进一步的研究表明，穿山龙总皂苷高、中、低剂量组均可增加外周血单个核细胞中 caspase-1、衔接凋亡相关斑点样蛋白含有半胱氨酸蛋白酶募集域（ASC）和 NALP3 的 mRNA 和蛋白水平（均 P<0.05）。
穿山龙可能通过调节溶酶体酶、抗氧化能力和 NALP3 炎性体来治疗 GA。
