Hu Yang, Teng Chunying, Yu Sumei, Wang Xin, Liang Jinsong, Bai Xin, Dong Liying, Song Tao, Yu Min, Qu Juanjuan
College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
School of Life Sciences, Tsinghua University, Beijing, 100084, People's Republic of China.
AMB Express. 2017 Dec;7(1):39. doi: 10.1186/s13568-017-0341-1. Epub 2017 Feb 14.
Polysaccharide is efficient in attenuation of metabolic ailments and modulation of gut microbiota as prebiotics. The therapeutic effect of Inonotus obliquus polysaccharide (IOP) on chronic pancreatitis (CP) in mice has been validated in our previous study. However, it is not clear whether IOP is conducive to maintaining the homeostasis between gut microbiota and host. The aim of this study is to testify the potential effects of IOP on gut microbiota composition and diversity in mice with CP. The changes in glutathione peroxidase (GSH-P), total antioxidant capacity (TAOC), tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), lipase and trypsin levels were measured by commercial assay kits, meanwhile the gut microbiota composition and diversity were analyzed by high throughput sequencing. The IOP treatment increased GSH-P and TAOC levels, and decreased TNF-α, TGF-β, lipase and trypsin levels in CP mice. It was also observed that gut microbiota in IOP treated groups were less diverse than others in terms of lower Shannon diversity index and Chao 1 estimator. IOP increased the proportion of Bacteroidetes and decreased that of Firmicutes at phylum level. Bacteroidetes was found positively correlated with GSH-P and TAOC, and Firmicutes correlated with TNF-α, TGF-β, and lipase. In conclusion, administration of IOP could regulate gut microbiota composition and diversity to a healthy profile in mice with CP, and some bacterial phylum significantly correlated with characteristic parameters.
多糖作为益生元，在减轻代谢疾病和调节肠道微生物群方面具有显著效果。我们之前的研究已经证实了桦褐孔菌多糖（IOP）对小鼠慢性胰腺炎（CP）的治疗作用。然而，目前尚不清楚IOP是否有助于维持肠道微生物群与宿主之间的稳态。本研究旨在验证IOP对CP小鼠肠道微生物群组成和多样性的潜在影响。通过商业检测试剂盒测定谷胱甘肽过氧化物酶（GSH-P）、总抗氧化能力（TAOC）、肿瘤坏死因子α（TNF-α）、转化生长因子β（TGF-β）、脂肪酶和胰蛋白酶水平的变化，同时通过高通量测序分析肠道微生物群的组成和多样性。IOP治疗提高了CP小鼠的GSH-P和TAOC水平，降低了TNF-α、TGF-β、脂肪酶和胰蛋白酶水平。还观察到，在香农多样性指数和Chao 1估计值较低的情况下，IOP治疗组的肠道微生物群比其他组的多样性更低。在门水平上，IOP增加了拟杆菌门的比例，降低了厚壁菌门的比例。发现拟杆菌门与GSH-P和TAOC呈正相关，厚壁菌门与TNF-α、TGF-β和脂肪酶呈正相关。总之，给予IOP可以将CP小鼠的肠道微生物群组成和多样性调节到健康状态，并且一些细菌门与特征参数显著相关。
