快速通讯：微卫星揭示亚马逊河下游巨骨舌鱼自然种群的遗传多样性与分化

RAPID-COMMUNICATION Genetic diversity and differentiation in natural populations of Arapaima gigas from lower Amazon revealed by microsatellites.

作者信息

Fazzi-Gomes P F, Melo N, Palheta G, Guerreiro S, Amador M, Ribeiro-Dos-Santos A K, Santos S, Hamoy I

机构信息

Laboratório de Genética Humana e Médica, Centro de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.

Laboratório de Genética Aplicada, Instituto Socioambiental e dos Recursos Hidricos, Universidade Federal Rural da Amazônia, Belém, PA, Brasil.

出版信息

Genet Mol Res. 2017 Feb 8;16(1):gmr-16-01-gmr.16019552. doi: 10.4238/gmr16019552.

DOI:10.4238/gmr16019552

PMID:28198511

Abstract

Genetic variability is one of the important criteria for species conservation decisions. This study aimed to analyze the genetic diversity and the population differentiation of two natural populations of Arapaima gigas, a species with a long history of being commercially exploited. We collected 87 samples of A. gigas from Grande Curuai Lake and Paru Lake, located in the Lower Amazon region of Amazônia, Brazil, and genotyped these samples using a multiplex panel of microsatellite markers. Our results showed that the populations of A. gigas analyzed had high levels of genetic variability, which were similar to those described in previous studies. These two populations had a significant population differentiation supported by the estimates of F and R (0.06), by Bayesian analysis (K = 2), and by population assignment tests, which revealed a moderate genetic distance.

摘要

遗传变异性是物种保护决策的重要标准之一。本研究旨在分析巨骨舌鱼两个自然种群的遗传多样性和种群分化，该物种长期以来一直遭受商业开发。我们从位于巴西亚马孙州下亚马孙地区的格兰德库拉伊湖和帕鲁湖采集了87份巨骨舌鱼样本，并使用微卫星标记多重面板对这些样本进行基因分型。我们的结果表明，所分析的巨骨舌鱼种群具有高水平的遗传变异性，这与先前研究中描述的相似。通过F和R估计值（0.06）、贝叶斯分析（K = 2）以及种群分配测试，这两个种群存在显著的种群分化，揭示出中等程度的遗传距离。