Park K-H, Cho E-H, Bae W-J, Kim H-S, Lim H-C, Park Y-D, Lee M-O, Cho E-S, Kim E-C
Department of Orthodontics, School of Dentistry, Kyung Hee University, Seoul, Korea.
Department of Oral and Maxillofacial Pathology, and Research Center for Tooth and Periodontal Regeneration (MRC), School of Dentistry, Kyung Hee University, Seoul, Korea.
J Periodontal Res. 2017 Jun;52(3):617-627. doi: 10.1111/jre.12430. Epub 2017 Feb 15.
Although expression of peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 (PIN1) was reported in bone tissue, the precise role of PIN1 in periodontal tissue and cells remain unclear.
MATERIAL & METHODS: To elucidate the roles of PIN1 in periodontal tissue, its expression in periodontal tissue and cells, and effects on in vitro 4 osteoblast differentiation and the underlying signaling mechanisms were evaluated.
PIN1 was expressed in mouse periodontal tissues including periodontal ligament cells (PDLCs), cementoblasts and osteoblasts at the developing root formation stage (postnatal, PN14) and functional stage of tooth (PN28). Treatment of PIN1 inhibitor juglone, and gene silencing by RNA interference promoted osteoblast differentiation in PDLCs and cementoblasts, whereas the overexpression of PIN1 inhibited. Moreover, osteogenic medium-induced activation of AMPK, mTOR, Akt, ERK, p38 and NF-jB pathways were enhanced by PIN1 siRNA, but attenuated by PIN1 overexpression. Runx2 expressions were induced by PIN1 siRNA, but downregulated by PIN1 overexpression.
In summary, this study is the first to demonstrate that PIN1 is expressed in developing periodontal tissue, and in vitro PDLCs and cementoblasts. PIN1 inhibition stimulates osteoblast differentiation, and thus may play an important role in periodontal regeneration.
尽管有报道称肽基脯氨酰顺/反异构酶NIMA相互作用蛋白1（PIN1）在骨组织中表达，但PIN1在牙周组织和细胞中的具体作用仍不清楚。
为阐明PIN1在牙周组织中的作用，评估了其在牙周组织和细胞中的表达，以及对体外成骨细胞分化的影响及其潜在的信号传导机制。
在牙根发育形成阶段（出生后，PN14）和牙齿功能阶段（PN28），PIN1在小鼠牙周组织中表达，包括牙周膜细胞（PDLCs）、成牙骨质细胞和成骨细胞。PIN1抑制剂胡桃醌处理以及RNA干扰介导的基因沉默促进了PDLCs和成牙骨质细胞的成骨细胞分化，而PIN1的过表达则起抑制作用。此外，PIN1 siRNA增强了成骨培养基诱导的AMPK、mTOR、Akt、ERK、p38和NF-κB信号通路的激活，但PIN1过表达则使其减弱。PIN1 siRNA诱导Runx2表达，但PIN1过表达使其下调。
总之，本研究首次证明PIN1在发育中的牙周组织以及体外培养的PDLCs和成牙骨质细胞中表达。PIN1抑制可刺激成骨细胞分化，因此可能在牙周再生中发挥重要作用。
