Jackman R W, Beeler D L, Fritze L, Soff G, Rosenberg R D
Proc Natl Acad Sci U S A. 1987 Sep;84(18):6425-9. doi: 10.1073/pnas.84.18.6425.
We have isolated a human thrombomodulin cDNA, and a human genomic clone containing the putative promoter domain, as well as the translated and untranslated regions of the endothelial cell receptor. The nucleotide sequence of the thrombomodulin cDNA allows us to provide a complete picture of the structure of this endothelial cell receptor, and to confirm its homology to the human low density lipoprotein receptor. The nucleotide sequence of the thrombomodulin gene suggests areas within the putative promoter domain that may be critical for regulating expression of the human endothelial cell receptor, indicates a potential signal peptide, and shows that no introns are present within the coding region. The overall organization of the human thrombomodulin gene is surprising because it represents an example of a gene that contains epidermal growth factor type B repeats and a membrane spanning region, which are not isolated within discrete exons.
我们分离出了人血栓调节蛋白cDNA，以及一个包含推定启动子结构域、内皮细胞受体的编码区和非编码区的人基因组克隆。血栓调节蛋白cDNA的核苷酸序列使我们能够全面了解这种内皮细胞受体的结构，并确认其与人低密度脂蛋白受体的同源性。血栓调节蛋白基因的核苷酸序列表明，推定启动子结构域内的某些区域可能对调节人内皮细胞受体的表达至关重要，显示出一个潜在的信号肽，并表明编码区内不存在内含子。人血栓调节蛋白基因的整体结构令人惊讶，因为它代表了一个包含B型表皮生长因子重复序列和跨膜区域的基因实例，这些区域并非孤立于离散的外显子中。
