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人血栓调节蛋白的结构与表达，一种内皮细胞上的凝血酶受体，作为蛋白C活化的辅因子。

Structure and expression of human thrombomodulin, a thrombin receptor on endothelium acting as a cofactor for protein C activation.

作者信息

Suzuki K, Kusumoto H, Deyashiki Y, Nishioka J, Maruyama I, Zushi M, Kawahara S, Honda G, Yamamoto S, Horiguchi S

机构信息

Department of Laboratory Medicine, Mie University School of Medicine, Japan.

出版信息

EMBO J. 1987 Jul;6(7):1891-7. doi: 10.1002/j.1460-2075.1987.tb02448.x.

DOI:10.1002/j.1460-2075.1987.tb02448.x
PMID:2820710
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553573/
Abstract

We have deduced the entire 575-amino acid sequence of the human thrombomodulin precursor from cDNA clones. The precursor starts with an 18-residue signal peptide domain, followed by the NH2-terminal domain, a domain with six epidermal growth factor-like structures, an O-glycosylation site-rich domain, a 24-residue transmembrane domain and a cytoplasmic domain. Simian COS cells transfected with the expression vector pSV2 containing thrombomodulin cDNA synthesized immunoreactive and functionally active thrombomodulin.

摘要

我们已从cDNA克隆中推导得到人血栓调节蛋白前体完整的575个氨基酸序列。该前体起始于一个18个残基的信号肽结构域，接着是NH2末端结构域、一个含有六个表皮生长因子样结构的结构域、一个富含O-糖基化位点的结构域、一个24个残基的跨膜结构域和一个胞质结构域。用含有血栓调节蛋白cDNA的表达载体pSV2转染的猴COS细胞合成了具有免疫反应性和功能活性的血栓调节蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/190f/553573/7ce82c27fc7f/emboj00247-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/190f/553573/61661adb9df8/emboj00247-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/190f/553573/7ce82c27fc7f/emboj00247-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/190f/553573/61661adb9df8/emboj00247-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/190f/553573/7ce82c27fc7f/emboj00247-0056-a.jpg

相似文献

1
Structure and expression of human thrombomodulin, a thrombin receptor on endothelium acting as a cofactor for protein C activation.人血栓调节蛋白的结构与表达，一种内皮细胞上的凝血酶受体，作为蛋白C活化的辅因子。
EMBO J. 1987 Jul;6(7):1891-7. doi: 10.1002/j.1460-2075.1987.tb02448.x.
2
Functional domains of membrane-bound human thrombomodulin. EGF-like domains four to six and the serine/threonine-rich domain are required for cofactor activity.膜结合型人血栓调节蛋白的功能结构域。辅因子活性需要表皮生长因子样结构域4至6以及富含丝氨酸/苏氨酸的结构域。
J Biol Chem. 1992 Mar 25;267(9):6164-70.
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Gene structure of human thrombomodulin, a thrombin receptor on endothelium acting as a cofactor for thrombin-catalyzed activation of protein C.人血栓调节蛋白的基因结构，它是内皮细胞上的一种凝血酶受体，作为凝血酶催化蛋白C活化的辅因子。
Nihon Ketsueki Gakkai Zasshi. 1988 Dec;51(8):1655-64.
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Microthrombomodulin. Residues 310-486 from the epidermal growth factor precursor homology domain of thrombomodulin will accelerate protein C activation.微血栓调节蛋白。来自血栓调节蛋白表皮生长因子前体同源结构域的310 - 486位残基将加速蛋白C的活化。
J Biol Chem. 1989 Feb 25;264(6):3352-6.
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Further localization of binding sites for thrombin and protein C in human thrombomodulin.凝血酶和蛋白C在人血栓调节蛋白中结合位点的进一步定位
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A domain composed of epidermal growth factor-like structures of human thrombomodulin is essential for thrombin binding and for protein C activation.人凝血酶调节蛋白中由表皮生长因子样结构组成的结构域对于凝血酶结合和蛋白C活化至关重要。
J Biol Chem. 1989 Mar 25;264(9):4872-6.
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Aspartic acid 349 in the fourth epidermal growth factor-like structure of human thrombomodulin plays a role in its Ca(2+)-mediated binding to protein C.人血栓调节蛋白第四表皮生长因子样结构中的天冬氨酸349在其钙介导的与蛋白C结合过程中发挥作用。
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The last three consecutive epidermal growth factor-like structures of human thrombomodulin comprise the minimum functional domain for protein C-activating cofactor activity and anticoagulant activity.人血栓调节蛋白的最后三个连续的表皮生长因子样结构构成了蛋白C激活辅因子活性和抗凝活性的最小功能域。
J Biol Chem. 1989 Jun 25;264(18):10351-3.
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Sequences required for thrombomodulin cofactor activity within the fourth epidermal growth factor-like domain of human thrombomodulin.人血栓调节蛋白第四表皮生长因子样结构域内血栓调节蛋白辅因子活性所需的序列。
J Biol Chem. 1993 Jul 15;268(20):15312-7.
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The function of calcium in protein C activation by thrombin and the thrombin-thrombomodulin complex can be distinguished by mutational analysis of protein C derivatives.通过对蛋白C衍生物进行突变分析，可以区分钙在凝血酶和凝血酶-血栓调节蛋白复合物激活蛋白C过程中的作用。
J Biol Chem. 1992 Dec 25;267(36):26104-9.

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The Role of the N-Terminal Domain of Thrombomodulin and the Potential of Recombinant Human Thrombomodulin as a Therapeutic Intervention for Shiga Toxin-Induced Hemolytic-Uremic Syndrome.血栓调节蛋白 N 端结构域的作用及重组人血栓调节蛋白作为志贺毒素诱导的溶血尿毒综合征治疗干预的潜力。
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Assessment of soluble thrombomodulin and soluble endoglin as endothelial dysfunction biomarkers in seriously ill surgical septic patients: correlation with organ dysfunction and disease severity.评估严重外科脓毒症患者内皮功能障碍的可溶性血栓调节蛋白和可溶性内皮糖蛋白标志物：与器官功能障碍和疾病严重程度的相关性。
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The pathogenesis and therapeutic strategies of heat stroke-induced liver injury.中暑性肝损伤的发病机制与治疗策略。
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Visualization of Domain- and Concentration-Dependent Impact of Thrombomodulin on Differential Regulation of Coagulation and Fibrinolysis.血栓调节蛋白对凝血和纤溶的差异化调节的域和浓度依赖性影响的可视化。
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Circulating Thrombomodulin: Release Mechanisms, Measurements, and Levels in Diseases and Medical Procedures.循环血栓调节蛋白：释放机制、检测方法以及在疾病和医疗程序中的水平
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Attempt for a Recombinant Thrombomodulin Alpha Treatment in a Rat Disseminated Intravascular Coagulation Model Using Yamakagashi () Venom.尝试使用山葵蛤（）毒液在大鼠弥散性血管内凝血模型中进行重组血栓调节蛋白α治疗。
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Establishing homologies in protein sequences.确定蛋白质序列中的同源性。
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Inactivation of human coagulation factor V by activated protein C.活化蛋白C对人凝血因子V的灭活作用。
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