Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University , FI-00076 Aalto, Finland.
Alabama Center for Paper and Bioresource Engineering, Department of Chemical Engineering, Auburn University , Auburn, Alabama 36849-5127, United States.
Biomacromolecules. 2017 Mar 13;18(3):898-905. doi: 10.1021/acs.biomac.6b01778. Epub 2017 Feb 27.
We present an efficient approach to develop cellulose nanocrystal (CNC) hybrids with magnetically responsive FeO nanoparticles that were synthesized using the (Fe/Fe) coprecipitation. After 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-catalyzed oxidation of CNC, carbodiimide (EDC/NHS) was used for coupling amine-containing iron oxide nanoparticles that were achieved by dopamine ligand exchange (NH-FeO NPs). The as-prepared hybrids (FeO@CNC) were further complexed with Cu(II) ions to produce specific protein binding sites. The performance of magnetically responsive Cu-FeO@CNC hybrids was assessed by selectively separating lysozyme from aqueous media. The hybrid system displayed a remarkable binding capacity with lysozyme of 860.6 ± 14.6 mg/g while near full protein recovery (∼98%) was achieved by simple elution. Moreover, the regeneration of FeO@CNC hybrids and efficient reutilization for protein separation was demonstrated. Finally, lysozyme separation from matrices containing egg white was achieved, thus revealing the specificity and potential of the presented method.
我们提出了一种有效的方法来开发纤维素纳米晶体（CNC）与磁响应的 FeO 纳米粒子的杂种，该纳米粒子是使用（Fe/Fe）共沉淀合成的。在 CNC 进行 2,2,6,6-四甲基哌啶-1-氧自由基（TEMPO）催化氧化后，使用碳二亚胺（EDC/NHS）偶联通过多巴胺配体交换（NH-FeO NPs）获得的含胺的氧化铁纳米粒子。通过将 Cu（II）离子进一步与所制备的杂化物（FeO@CNC）络合，生成具有特定蛋白质结合位点的杂化物。通过从水介质中选择性分离溶菌酶来评估磁响应的 Cu-FeO@CNC 杂种的性能。该混合体系对溶菌酶具有显著的结合能力，结合能力为 860.6 ± 14.6 mg/g，而通过简单洗脱几乎可以实现近 100%的蛋白质回收率（约 98%）。此外，还证明了 FeO@CNC 杂种的再生和蛋白质分离的有效再利用。最后，实现了从含有蛋清的基质中分离溶菌酶，从而揭示了所提出方法的特异性和潜力。
