Patwa Rahul, Zandraa Oyunchimeg, Capáková Zdenka, Saha Nabanita, Sáha Petr
Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Tř. T. Bati 5678, 760 01 Zlín, Czech Republic.
Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech Republic.
Polymers (Basel). 2020 Nov 14;12(11):2690. doi: 10.3390/polym12112690.
In this study we report the preparation of novel multicomponent hydrogels as potential biomaterials for injectable hydrogels comprised of alginate, casein and bacterial cellulose impregnated with iron nanoparticles (BCF). These hydrogels demonstrated amide cross-linking of alginate-casein, ionic cross-linking of alginate and supramolecular interaction due to incorporation of BCF. Incorporation of BCF into the hydrogels based on natural biopolymers was done to reinforce the hydrogels and impart magnetic properties critical for targeted drug delivery. This study aimed to improve overall properties of alginate/casein hydrogels by varying the BCF loading. The physico-chemical properties of gels were characterized via FTIR, XRD, DSC, TGA, VSM and mechanical compression. In addition, swelling, drug release, antibacterial activity and cytotoxicity studies were also conducted on these hydrogels. The results indicated that incorporation of BCF in alginate/casein hydrogels led to mechanically stronger gels with magnetic properties, increased porosity and hence increased swelling. A porous structure, which is essential for migration of cells and biomolecule transportation, was confirmed from microscopic analysis. The porous internal structure promoted cell viability, which was confirmed through MTT assay of fibroblasts. Moreover, a hydrogel can be useful for the delivery of essential drugs or biomolecules in a sustained manner for longer durations. These hydrogels are porous, cell viable and possess mechanical properties that match closely to the native tissue. Collectively, these hybrid alginate-casein hydrogels laden with BCF can be fabricated by a facile approach for potential wound healing applications.
在本研究中，我们报告了新型多组分水凝胶的制备，该水凝胶作为可注射水凝胶的潜在生物材料，由海藻酸盐、酪蛋白和浸渍有铁纳米颗粒（BCF）的细菌纤维素组成。这些水凝胶表现出海藻酸盐 - 酪蛋白的酰胺交联、海藻酸盐的离子交联以及由于BCF的掺入而产生的超分子相互作用。将BCF掺入基于天然生物聚合物的水凝胶中，是为了增强水凝胶并赋予对靶向药物递送至关重要的磁性。本研究旨在通过改变BCF负载量来改善海藻酸盐/酪蛋白水凝胶的整体性能。通过傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、差示扫描量热法（DSC）、热重分析（TGA）、振动样品磁强计（VSM）和机械压缩对凝胶的物理化学性质进行了表征。此外，还对这些水凝胶进行了溶胀、药物释放、抗菌活性和细胞毒性研究。结果表明，在海藻酸盐/酪蛋白水凝胶中掺入BCF会导致具有磁性、机械强度更高、孔隙率增加从而溶胀增加的凝胶。微观分析证实了多孔结构对于细胞迁移和生物分子运输至关重要。通过成纤维细胞的MTT测定证实，多孔内部结构促进了细胞活力。此外，水凝胶可用于持续更长时间递送必需药物或生物分子。这些水凝胶具有多孔性、细胞活性且具有与天然组织紧密匹配的机械性能。总体而言，这些负载BCF的海藻酸盐 - 酪蛋白杂化水凝胶可以通过简便的方法制备，用于潜在的伤口愈合应用。
