Dörries K, Loeber G, Meixensberger J
Virology. 1987 Sep;160(1):268-70. doi: 10.1016/0042-6822(87)90071-7.
Human brain tumors of 11 different types were analyzed by Southern blot analysis for the presence of JCV, SV40, and BKV. In 21 tumor specimens examined with JCV- and SV40-specific probes no positive hybridizations were obtained. Analysis for BKV DNA, however, revealed the presence of BKV-specific sequences in 11 of 24 tumor specimens. No hybridization was found in DNA from CNS tissues from different areas of 29 individuals without CNS tumors. The BKV DNA sequences were associated with high molecular weight cellular DNA, suggesting a chromosomal location. These data provide evidence for the involvement of BKV in the development of human brain tumors.
通过Southern印迹分析，对11种不同类型的人脑肿瘤进行检测，以确定是否存在JC病毒（JCV）、猿猴病毒40（SV40）和BK病毒（BKV）。在用JCV和SV40特异性探针检测的21个肿瘤标本中，未获得阳性杂交信号。然而，对BKV DNA的分析显示，在24个肿瘤标本中的11个中存在BKV特异性序列。在来自29名无中枢神经系统肿瘤个体不同区域的中枢神经系统组织DNA中未发现杂交信号。BKV DNA序列与高分子量细胞DNA相关，提示其定位于染色体。这些数据为BKV参与人脑肿瘤的发生发展提供了证据。
