Kim John Y H, Koralnik Igor J, LeFave Mark, Segal Rosalind A, Pfister Luz-Andrea, Pomeroy Scott L
Division of Neuroscience, Department of Neurology, Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA.
Neuro Oncol. 2002 Jul;4(3):165-70. doi: 10.1093/neuonc/4.3.165.
To address the hypothesis that medulloblastoma or supratentorial primitive neuroectodermal tumor (sPNET) can arise through infection by polyomaviruses, we examined genomic DNA isolated from 15 primary medulloblastoma and 5 sPNET biopsy specimens and from 2 medulloblastoma cell lines for the presence of DNA sequences from the polyomaviruses simian virus 40 (SV40), JC virus, and BK virus. These polyomaviruses have oncogenic potential in animals, and their DNA sequences have been detected in other surveys of various solid tumors, including childhood brain tumors. The tumor DNA samples were analyzed by Southern blot hybridization of polymerase chain reaction products that employed probes designed to detect specific polyomavirus sequences. Neither JC virus nor BK virus DNA sequences were detected in any of the specimens. None of the primary medulloblastoma or sPNET specimens contained SV40 sequences. However, SV40 DNA coding and noncoding sequences were detected in the D283-Med (medulloblastoma) cell line. Immunocytochemical studies of D283-Med revealed nuclear expression of SV40 large T antigen. In contrast to childhood ependymomas and choroid plexus tumors, medulloblastomas and sPNETs infrequently express evidence of polyomavirus infection.
为了验证髓母细胞瘤或幕上原始神经外胚层肿瘤（sPNET）可能由多瘤病毒感染引起这一假说，我们检测了从15份原发性髓母细胞瘤活检标本、5份sPNET活检标本以及2株髓母细胞瘤细胞系中分离出的基因组DNA，以查找来自多瘤病毒猴病毒40（SV40）、JC病毒和BK病毒的DNA序列。这些多瘤病毒在动物体内具有致癌潜力，并且在包括儿童脑肿瘤在内的各种实体瘤的其他调查中已检测到它们的DNA序列。通过对聚合酶链反应产物进行Southern印迹杂交来分析肿瘤DNA样本，该反应采用了设计用于检测特定多瘤病毒序列的探针。在任何标本中均未检测到JC病毒或BK病毒的DNA序列。原发性髓母细胞瘤或sPNET标本中均未包含SV40序列。然而，在D283-Med（髓母细胞瘤）细胞系中检测到了SV40 DNA编码和非编码序列。对D283-Med进行的免疫细胞化学研究显示SV40大T抗原的核表达。与儿童室管膜瘤和脉络丛肿瘤不同，髓母细胞瘤和sPNET很少表现出多瘤病毒感染的迹象。
