Wood W G, Lahiri S, Gorka C, Armbrecht H J, Strong R
Alcohol Clin Exp Res. 1987 Aug;11(4):332-5. doi: 10.1111/j.1530-0277.1987.tb01320.x.
Chronic ethanol consumption has been shown to affect physical properties of membranes from animals as measured by electron spin resonance (ESR). This study compared for the first time the physical properties of erythrocyte membranes of alcoholic patients and control subjects using ESR procedures. Membrane fluidity was determined in the presence and absence of ethanol using the 5-doxyl stearic acid spin-label. Temperature-dependent phase transition also was determined, as were comparisons between ESR parameters, at the 1st and 5th week after alcohol withdrawal. Ethanol-induced fluidity was significantly greater in membranes of control subjects compared with alcoholic patients. Baseline fluidity did not differ and the temperature at which the phase transition occurred was not significantly different between the two groups. The resistance of membranes of alcoholic patients to fluidization by ethanol was unchanged after 5 weeks of withdrawal. Comparisons between ethanol-induced fluidization at the 1st and 5th week after withdrawal were not significantly different. These studies demonstrate differences in ethanol-induced fluidization between alcoholic patients and control subjects that are consistent with earlier ESR studies using an animal model.
慢性乙醇摄入已被证明会影响动物细胞膜的物理性质，这是通过电子自旋共振（ESR）测量得出的。本研究首次使用ESR程序比较了酒精性患者和对照受试者红细胞膜的物理性质。使用5-硬脂酸自旋标记物在有和没有乙醇的情况下测定膜流动性。还测定了温度依赖性相变，并比较了戒酒第1周和第5周时的ESR参数。与酒精性患者相比，对照受试者的膜中乙醇诱导的流动性明显更大。两组的基线流动性没有差异，相变发生的温度也没有显著差异。戒酒5周后，酒精性患者的膜对乙醇流化的抗性没有变化。戒酒后第1周和第5周乙醇诱导的流化之间的比较没有显著差异。这些研究表明，酒精性患者和对照受试者之间乙醇诱导的流化存在差异，这与早期使用动物模型的ESR研究一致。
