Benedetti A, Birarelli A M, Brunelli E, Curatola G, Ferretti G, Jezequel A M, Orlandi F
Pharmacol Res Commun. 1986 Nov;18(11):1003-14. doi: 10.1016/0031-6989(86)90018-4.
Chronic alcoholics (greater than 150 g/day) showing minor serum and histological changes have been studied, compared to healthy non alcoholic subjects, and the following parameters have been considered: S. (Serum)cholesterol (CH), S.phospholipids (PH), S.folate level, and mean corpuscular volume (MCV). Erythrocyte ghosts have been studied for CH and PH content and membrane fluidity using diphenylhexatriene as a probe. All alcoholics showed decreased fluidity of red cell membrane with increased CH/PH ratio, even in patients showing normal MCV or minimal alterations of functional tests, suggesting that changes in red cell membrane fluidity represent an early sign of ethanol abuse. These likely reflect the diffuse interaction of ethanol with biological membranes. The administration of N5-Methyltetrahydrofolate produced an increase of membrane fluidity over the 3 weeks considered, associated with modest changes of MCV. The latter were delayed with respect to changes in fluidity. If changes of red cell membrane fluidity are a sensitive index of alcohol abuse, they could be a useful marker for detection and follow-up of chronic alcoholism.
对每日饮酒量超过150克、血清和组织学有轻微变化的慢性酗酒者进行了研究，并与健康的非酗酒者进行了比较，同时考虑了以下参数：血清胆固醇（CH）、血清磷脂（PH）、血清叶酸水平和平均红细胞体积（MCV）。以二苯基己三烯为探针，研究了红细胞影的CH和PH含量以及膜流动性。所有酗酒者即使在MCV正常或功能测试仅有轻微改变的患者中，也表现出红细胞膜流动性降低，CH/PH比值升高，这表明红细胞膜流动性的改变是乙醇滥用的早期迹象。这些可能反映了乙醇与生物膜的广泛相互作用。在为期3周的观察期内，给予N5-甲基四氢叶酸可使膜流动性增加，同时MCV有适度变化。后者相对于流动性变化有所延迟。如果红细胞膜流动性的改变是酒精滥用的敏感指标，那么它们可能是检测和随访慢性酒精中毒的有用标志物。
