Hopiavuori Blake R, Agbaga Martin-Paul, Brush Richard S, Sullivan Michael T, Sonntag William E, Anderson Robert E
Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.
Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104; Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104; Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104; Dean McGee Eye Institute, Oklahoma City, OK 73104.
J Lipid Res. 2017 Apr;58(4):668-680. doi: 10.1194/jlr.M070714. Epub 2017 Feb 15.
We present here a quantitative molecular blueprint of the three major glycerophospholipid (GPL) classes, phosphatidylcholine (PC), phosphatidylserine (PS), and phosphatidylethanolamine (PE), in retina and six regions of the brain in C57Bl6 mice at 2, 10, and 26 months of age. We found an age-related increase in molecular species containing saturated and monoenoic FAs and an overall decrease in the longer-chain PUFA molecular species across brain regions, with loss of DHA-containing molecular species as the most consistent and dramatic finding. Although we found very-long-chain PUFAs (VLC-PUFAs) (C28) in PC in the retina, no detectable levels were found in any brain region at any of the ages examined. All brain regions (except hippocampus and retina) showed a significant increase with age in PE plasmalogens. All three retina GPLs had di-PUFA molecular species (predominantly 44:12), which were most abundant in PS (∼30%). In contrast, low levels of di-PUFA GPL (1-2%) were found in all regions of the brain. This study provides a regional and age-related assessment of the brain's lipidome with a level of detail, inclusion, and quantification that has not heretofore been published.
我们在此展示了C57Bl6小鼠在2个月、10个月和26个月大时，视网膜及大脑六个区域中三种主要甘油磷脂（GPL）类别，即磷脂酰胆碱（PC）、磷脂酰丝氨酸（PS）和磷脂酰乙醇胺（PE）的定量分子蓝图。我们发现，在所有脑区中，含饱和脂肪酸和单不饱和脂肪酸的分子种类随年龄增长而增加，而长链多不饱和脂肪酸分子种类总体减少，其中含DHA的分子种类减少最为一致且显著。尽管我们在视网膜的PC中发现了极长链多不饱和脂肪酸（VLC-PUFAs）（≥C28），但在所检测的任何年龄的任何脑区均未检测到其存在。所有脑区（海马体和视网膜除外）的PE缩醛磷脂均随年龄增长而显著增加。视网膜中的所有三种GPL均含有二多不饱和脂肪酸分子种类（主要为44:12），其中在PS中含量最为丰富（约30%）。相比之下，在大脑的所有区域中，二多不饱和脂肪酸GPL的含量较低（1-2%）。本研究提供了对大脑脂质组的区域和年龄相关评估，其详细程度、涵盖范围和定量水平均为前所未发表。
