Lopez A, Yong S, Sharma A, Prior M
Am J Vet Res. 1987 Aug;48(8):1282-5.
The effect of vehicular saline solution volume on early lesions induced in rats by intratracheal administration of silica was evaluated. Seventy-two male Long-Evans rats were randomly assigned 6 each to 12 factorial groups (3 X 2 X 2): 3 doses of silica (0, 2.5, and 5 mg), 2 volumes of vehicle (saline solution; 0.1 and 0.5 ml), and 2 postinoculation times (1 and 3 days). Lactate dehydrogenase and alkaline phosphatase activities in the bronchoalveolar lavage fluid supernatant and cell viability of bronchoalveolar cells were used as indicators of cell injury. The number of pulmonary alveolar macrophages and polymorphonuclear leukocytes were used as indicators of inflammatory response. Dose of silica and postinoculation time had a significant (P less than 0.05) effect on the biochemical and cellular composition of lavage fluid. The volume of vehicle in which silica was suspended significantly (P less than 0.05) enhanced the pulmonary injury and inflammatory response. However, dose-volume interaction was only significant (P less than 0.05) in 1 of 6 parameters, indicating that the effect was additive, but not synergistic, in nature. Seemingly, vehicle volume had an enhanced effect on the injury and the inflammatory response induced by intratracheal inoculation of silica.
评估了气管内给予二氧化硅后，载体盐溶液体积对大鼠早期损伤的影响。72只雄性Long-Evans大鼠被随机分为12个析因组，每组6只（3×2×2）：3个二氧化硅剂量（0、2.5和5毫克）、2个载体体积（盐溶液；0.1和0.5毫升）以及2个接种后时间（1天和3天）。支气管肺泡灌洗流体上清液中的乳酸脱氢酶和碱性磷酸酶活性以及支气管肺泡细胞的细胞活力被用作细胞损伤的指标。肺泡巨噬细胞和多形核白细胞的数量被用作炎症反应的指标。二氧化硅剂量和接种后时间对灌洗流体的生化和细胞组成有显著（P<0.05）影响。悬浮二氧化硅的载体体积显著（P<0.05）增强了肺损伤和炎症反应。然而，剂量-体积相互作用仅在6个参数中的1个中显著（P<0.05），表明其作用本质上是相加的，而非协同的。显然，载体体积对气管内接种二氧化硅诱导的损伤和炎症反应有增强作用。
