Woldekidan Samuel, Mulu Abay, Ergetie Wondwossen, Teka Frehiwot, Meressa Asfaw, Tadele Ashenif, Abebe Abiy, Gemechu Worku, Gemeda Negero, Ashebir Rekik, Sileshi Meron, Tolcha Yoseph
Directorate of Traditional and Modern Medicine Research, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Department of Anatomy, School of Medicine, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia.
Diabetes Metab Syndr Obes. 2021 Jan 15;14:185-192. doi: 10.2147/DMSO.S266794. eCollection 2021.
Diabetes is a serious metabolic disorder with complications that result in significant morbidity and mortality. Current drugs used for diabetes therapy are not free from side effects and do not restore normal glucose homeostasis. Therefore, the purpose of this study is to evaluate the antidiabetic effect of (Baker f.) aqueous leaves extract.
Thirty rats of weight 90-150 gram were distributed to five groups (n= 6). Then labelled as diabetic control (DC), normal control (NC), extract treated (MS 250 and 500mg/kg), and glibenclamide treated (GL 5mg/kg). The experimental rats were induced by intra-peritoneal injection of Alloxan monohydrate at a dose of 180 mg/kg after dissolving in normal saline. Clinical biochemistry such as AST, ALT, ALP, urea, creatinine, and cholesterol, blood glucose level, histopathological and preliminary phytochemical screening were evaluated.
Phytochemical tests revealed the presence of different secondary metabolites. Alkaloid, flavonoid, tannin, saponin, phytosteroids, phenols and terpenoids. (Baker f.) leaves aqueous extract (250 and 500mg/kg) improved the body weight of rats, showed remarkable reduction in blood glucose concentration (P<0.05), and significantly decreased serum urea, creatinine, ALT, AST and ALP (P < 0.05). Levels of serum cholesterol remained unaltered in the experimental groups when compared with diabetic control. Histopathology of non-treated rats showed deterioration of insulin producing pancreas cells; nevertheless, β-cells restoration was observed due to administration of (Baker f.) aqueous leaves extract.
It is possible to conclude that oral administration of (Baker f.) aqueous leaf extracts (250mg/kg and 500mg/kg) for 28 days showed beneficial effects on antihyperglycemia, improved body weight and Alloxan damaged pancreatic β-cells, and restored biochemical changes.
糖尿病是一种严重的代谢紊乱疾病，其并发症会导致显著的发病率和死亡率。目前用于糖尿病治疗的药物并非没有副作用，也无法恢复正常的葡萄糖稳态。因此，本研究的目的是评估（贝克氏）叶水提取物的抗糖尿病作用。
将30只体重90 - 150克的大鼠分为五组（n = 6）。然后标记为糖尿病对照组（DC）、正常对照组（NC）、提取物处理组（MS 250和500mg/kg）以及格列本脲处理组（GL 5mg/kg）。将实验大鼠腹腔注射溶解于生理盐水中的一水合四氧嘧啶，剂量为180mg/kg。评估临床生化指标如谷草转氨酶（AST）、谷丙转氨酶（ALT）、碱性磷酸酶（ALP）、尿素、肌酐和胆固醇、血糖水平、组织病理学以及初步的植物化学筛选。
植物化学测试显示存在不同的次生代谢产物。生物碱、黄酮类、单宁、皂苷、植物甾醇、酚类和萜类。（贝克氏）叶水提取物（250和500mg/kg）改善了大鼠体重，血糖浓度显著降低（P < 0.05），血清尿素、肌酐、ALT、AST和ALP显著下降（P < 0.05）。与糖尿病对照组相比，实验组血清胆固醇水平未发生改变。未处理大鼠的组织病理学显示胰岛素产生胰腺细胞恶化；然而，由于给予（贝克氏）叶水提取物，观察到β细胞恢复。
可以得出结论，口服（贝克氏）叶水提取物（250mg/kg和500mg/kg）28天对抗高血糖、改善体重以及四氧嘧啶损伤的胰腺β细胞具有有益作用，并恢复了生化变化。
