Ma Rong, Jiang Dongmei, Kang Bo, Bai Lin, He Hui, Chen Ziyu, Yi Zhixin
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Gene. 2015 Aug 15;568(1):55-60. doi: 10.1016/j.gene.2015.05.014. Epub 2015 May 7.
Antizyme inhibitor 1 (Azin1) plays critical roles in various cellular pathways, including ornithine decarboxylase regulation, polyamine anabolism and uptake and cell proliferation. However, the molecular characteristics of the AZIN1 gene and its expression profile in goose tissues and ovarian follicles have not been reported. In this study, the AZIN1 cDNA of the Sichuan white goose (Anser cygnoides) was cloned, and analyzed for its phylogenetic and physiochemical properties. The expression profile of AZIN1 mRNA in geese tissues and ovarian follicles were examined using quantitative real-time PCR. The results showed that the open reading frame of the AZIN1 cDNA is 1,353 bp in length, encoding a 450 amino acid protein with a molecular weight of 50 kDa. Out of all tissues examined, AZIN1 expression was highest in the adrenal gland and lowest in breast muscle. There was also a high expression of AZIN1 in the cerebellum and isthmus of oviduct. With follicular development, AZIN1 gene expression gradually increased, and its expression in F1 was significantly higher than in F5 (P<0.05). AZIN1 expression was also significantly higher in the POF1 than in the other follicles (P<0.05), and there was a low mRNA expression of AZIN1 in atretic follicles. The results of AZIN1 expression profiling in ovarian follicles suggest that AZIN1 may play an important role in the progression of follicular development, potentially through regulating polyamine levels.
抗酶抑制剂1（Azin1）在多种细胞途径中发挥关键作用，包括鸟氨酸脱羧酶调节、多胺合成代谢与摄取以及细胞增殖。然而，AZIN1基因的分子特征及其在鹅组织和卵泡中的表达谱尚未见报道。本研究克隆了四川白鹅（Anser cygnoides）的AZIN1 cDNA，并分析了其系统发育和理化性质。采用定量实时PCR检测了AZIN1 mRNA在鹅组织和卵泡中的表达谱。结果显示，AZIN1 cDNA的开放阅读框长度为1353 bp，编码一个由450个氨基酸组成、分子量为50 kDa的蛋白质。在所检测的所有组织中，AZIN1在肾上腺中的表达最高，在胸肌中的表达最低。AZIN1在小脑和输卵管峡部也有高表达。随着卵泡发育，AZIN1基因表达逐渐增加，其在F1中的表达显著高于F5（P<0.05）。AZIN1在POF1中的表达也显著高于其他卵泡（P<0.05），而在闭锁卵泡中AZIN1的mRNA表达较低。卵泡中AZIN1表达谱的结果表明，AZIN1可能通过调节多胺水平在卵泡发育进程中发挥重要作用。
