Tian Kai, Xiao Qihai, Zhang Xueyou, Lan Xi, Zhao Xiaoling, Wang Yan, Li Diyan, Yin Huadong, Ye Lin, Zhu Qing
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, 611130, China.
Funct Integr Genomics. 2017 Nov;17(6):687-696. doi: 10.1007/s10142-017-0564-x. Epub 2017 Jun 14.
GPR133 (G protein-coupled receptor 133) plays significant roles in various physiological processes. Alternatively splicing (AS) variants of GPR133 in many species have been predicted in multiple databases, but there is no available information about the AS events of chicken GPR133 (cGPR133). In the present study, two chicken GPR133 variants, cGPR133-va and cGPR133-vb, were identified by a combination of reverse transcription PCR (RT-PCR) and rapid amplification of cDNA 5'-ends (5' RACE). Sequence analysis shows that cGPR133-va and cGPR133-vb are resulting from different AS modules and their sequences are predicted to encode two distinct putative proteins, respectively. Quantitative real-time PCR (qRT-PCR) analysis revealed that cGPR133-va and cGPR133-vb are widely expressed in different tissues, while exhibiting specific expression profile. Altogether, our results first demonstrate the existence of novel cGPR133 variants and illustrate its transcriptional diversity and their widespread distribution, which provides a foundation for the further research of GPR133.
GPR133（G蛋白偶联受体133）在多种生理过程中发挥着重要作用。多个数据库已预测出许多物种中GPR133的可变剪接（AS）变体，但关于鸡GPR133（cGPR133）的AS事件尚无可用信息。在本研究中，通过逆转录PCR（RT-PCR）和cDNA 5'末端快速扩增（5' RACE）相结合的方法，鉴定出两种鸡GPR133变体，即cGPR133-va和cGPR133-vb。序列分析表明，cGPR133-va和cGPR133-vb源自不同的AS模块，并且它们的序列预计分别编码两种不同的假定蛋白质。定量实时PCR（qRT-PCR）分析显示，cGPR133-va和cGPR133-vb在不同组织中广泛表达，同时呈现出特定的表达谱。总之，我们的结果首次证明了新型cGPR133变体的存在，并阐明了其转录多样性及其广泛分布，这为进一步研究GPR133奠定了基础。
