Cullinan W E, Brunjes P C
Neuroscience Program, University of Virginia, Charlottesville 22903.
Brain Res. 1987 Sep;432(1):35-42. doi: 10.1016/0165-3806(87)90006-x.
Littermate rat pups underwent either unilateral surgical occlusion of the right external naris or sham surgery on postnatal Day 1. At 4-, 8-, 12-, 20- or 30-days postpartum, olfactory bulb sections were histochemically reacted to reveal either succinate dehydrogenase (SDH) or cytochrome oxidase (CO) activity. Microdensitometry was used to determine levels of staining in glomeruli and the external plexiform layer at standardized locations within the bulb. In experimental subjects asymmetries in left/right bulb SDH staining patterns were detected as early as Day 4, suggesting that the deprivation procedure resulted in quite rapid changes in the metabolic function of olfactory bulb cells. Control animals did not exhibit left/right differences in bulb staining, but inter-glomerular and regional variations in staining were observed throughout the early developmental period. Understanding these early variations in metabolic activity may be important for a complete understanding of olfactory bulb maturation.
同窝出生的幼鼠在出生后第1天接受了右侧外鼻孔单侧手术闭塞或假手术。在产后4天、8天、12天、20天或30天，对嗅球切片进行组织化学反应，以显示琥珀酸脱氢酶（SDH）或细胞色素氧化酶（CO）的活性。使用显微密度测定法来确定嗅球内标准化位置处肾小球和外丛状层的染色水平。在实验对象中，早在第4天就检测到左右嗅球SDH染色模式的不对称，这表明剥夺程序导致嗅球细胞代谢功能发生相当迅速的变化。对照动物的嗅球染色没有表现出左右差异，但在整个早期发育阶段观察到了肾小球间和区域染色变化。了解这些代谢活动的早期变化对于全面理解嗅球成熟可能很重要。
