ISIS, Institut de Science et d'Ingénierie Supramoléculaires , 8 allée Gaspard Monge, 67000 Strasbourg, France.
SAMS Research Group, University of Strasbourg , Institut Charles Sadron, CNRS, 23 rue du Loess, BP 84047, 67034 Strasbourg Cedex 2, France.
J Am Chem Soc. 2017 Mar 15;139(10):3721-3727. doi: 10.1021/jacs.6b12094. Epub 2017 Mar 2.
Triarylamine molecules appended with crown-ethers or carboxylic moieties form self-assembled supramolecular channels within lipid bilayers. Fluorescence assays and voltage clamp studies reveal that the self-assemblies incorporating the crown ethers work as single channels for the selective transport of K or Rb. The X-ray crystallographic structures confirm the mutual columnar self-assembly of triarylamines and crown-ethers. The dimensional fit of K cations within the 18-crown-6 leads to a partial dehydration and to the formation of alternating K cation-water wires within the channel. This original type of organization may be regarded as a biomimetic alternative of columnar K-water wires observed for the natural KcsA channel. Supramolecular columnar arrangement was also shown for the triarylamine-carboxylic acid conjugate. In this latter case, stopped-flow light scattering analysis reveals the transport of water across lipid bilayer membranes with a relative water permeability as high as 17 μm s.
三芳胺分子连接冠醚或羧酸部分，在双层脂膜内形成自组装超分子通道。荧光测定和电压钳研究表明，包含冠醚的自组装体可以作为单通道，选择性地运输 K 或 Rb。X 射线晶体结构证实了三芳胺和冠醚的相互柱状自组装。K 阳离子在 18-冠-6 内的尺寸适合导致部分脱水，并在通道内形成交替的 K 阳离子-水线。这种组织形式可能被视为天然 KcsA 通道中观察到的柱状 K-水线的仿生替代。超分子柱状排列也显示在三芳胺-羧酸缀合物中。在后一种情况下，停流光散射分析显示水跨脂质双层膜的转运，相对水渗透率高达 17μm/s。
