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电鳐乙酰胆碱酯酶的cDNA序列：催化亚基前体的一级结构；多个5'-非翻译区的存在

cDNA sequences of Torpedo marmorata acetylcholinesterase: primary structure of the precursor of a catalytic subunit; existence of multiple 5'-untranslated regions.

作者信息

Sikorav J L, Krejci E, Massoulié J

机构信息

Laboratoire de Neurobiologie, Ecole Normale Supérieure, Paris, France.

出版信息

EMBO J. 1987 Jul;6(7):1865-73. doi: 10.1002/j.1460-2075.1987.tb02445.x.

DOI:10.1002/j.1460-2075.1987.tb02445.x
PMID:2820709
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553570/
Abstract

cDNA clones coding for a catalytic subunit of acetylcholinesterase were isolated from cDNA libraries constructed from Torpedo marmorata electric organ. The nucleotide sequence of the cloned cDNAs codes for a 599-amino acid precursor containing a 24-amino acid signal peptide. This primary structure has been compared with the sequences of Torpedo californica and Drosophila melanogasta acetylcholinesterases, and with that of human butyrylcholinesterase. Genomic blot experiments carried out with cDNA restriction fragments used as hybridization probes are in agreement with the existence of a single gene coding for the different catalytic subunits of Torpedo acetylcholinesterase. Unexpectedly, we observed multiple 5'-untranslated regions, which may contain several initiation codons.

摘要

从电鳐（Torpedo marmorata）电器官构建的cDNA文库中分离出编码乙酰胆碱酯酶催化亚基的cDNA克隆。克隆的cDNA的核苷酸序列编码一个含有24个氨基酸信号肽的599个氨基酸的前体。该一级结构已与加州电鳐（Torpedo californica）和黑腹果蝇（Drosophila melanogasta）乙酰胆碱酯酶的序列以及人丁酰胆碱酯酶的序列进行了比较。用cDNA限制性片段作为杂交探针进行的基因组印迹实验与存在一个编码电鳐乙酰胆碱酯酶不同催化亚基的单一基因的情况一致。出乎意料的是，我们观察到多个5'-非翻译区，其可能包含几个起始密码子。

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cDNA sequences of Torpedo marmorata acetylcholinesterase: primary structure of the precursor of a catalytic subunit; existence of multiple 5'-untranslated regions.电鳐乙酰胆碱酯酶的cDNA序列：催化亚基前体的一级结构；多个5'-非翻译区的存在
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本文引用的文献

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Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.淋巴细胞有丝分裂原和血小板衍生生长因子对c-myc基因的细胞特异性调控。
Cell. 1983 Dec;35(3 Pt 2):603-10. doi: 10.1016/0092-8674(83)90092-2.
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Structural organization of the 3' half of the rat thyroglobulin gene.大鼠甲状腺球蛋白基因3'端一半的结构组织
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A single gene codes for the nicotinic acetylcholine receptor alpha-subunit in Torpedo marmorata: structural and developmental implications.
一大类酯酶、脂肪酶及相关蛋白质中序列保守性与三维结构之间的关系。
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Neural regulation of acetylcholinesterase mRNAs at mammalian neuromuscular synapses.哺乳动物神经肌肉突触处乙酰胆碱酯酶mRNA的神经调节。
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Brain cDNA clone for human cholinesterase.人胆碱酯酶的脑cDNA克隆。
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6682-6. doi: 10.1073/pnas.84.19.6682.
6
An asymmetric form of muscle acetylcholinesterase contains three subunit types and two enzymic activities in one molecule.肌肉乙酰胆碱酯酶的一种不对称形式在一个分子中包含三种亚基类型和两种酶活性。
Proc Natl Acad Sci U S A. 1988 Feb;85(4):1262-6. doi: 10.1073/pnas.85.4.1262.
7
Complex alternative splicing of acetylcholinesterase transcripts in Torpedo electric organ; primary structure of the precursor of the glycolipid-anchored dimeric form.电鳐电器官中乙酰胆碱酯酶转录本的复杂可变剪接；糖脂锚定二聚体形式前体的一级结构。
EMBO J. 1988 Oct;7(10):2983-93. doi: 10.1002/j.1460-2075.1988.tb03161.x.
8
Allelic variants of acetylcholinesterase: genetic evidence that all acetylcholinesterase forms in avian nerves and muscles are encoded by a single gene.乙酰胆碱酯酶的等位基因变体：鸟类神经和肌肉中所有乙酰胆碱酯酶形式均由单一基因编码的遗传学证据。
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7805-9. doi: 10.1073/pnas.85.20.7805.
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Tissue-specific processing and polarized compartmentalization of clone-produced cholinesterase in microinjected Xenopus oocytes.克隆产生的胆碱酯酶在显微注射的非洲爪蟾卵母细胞中的组织特异性加工和极化区室化
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
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Antigenic and structural differences in the catalytic subunits of the molecular forms of acetylcholinesterase.乙酰胆碱酯酶分子形式催化亚基的抗原性和结构差异。
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The molecular forms of cholinesterase and acetylcholinesterase in vertebrates.脊椎动物体内胆碱酯酶和乙酰胆碱酯酶的分子形式。
Annu Rev Neurosci. 1982;5:57-106. doi: 10.1146/annurev.ne.05.030182.000421.
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Synthesis in vitro of precursors of the catalytic subunits of acetylcholinesterase from Torpedo marmorata and Electrophorus electricus.电鳐和电鳗乙酰胆碱酯酶催化亚基前体的体外合成
Eur J Biochem. 1984 Dec 17;145(3):519-24. doi: 10.1111/j.1432-1033.1984.tb08587.x.
10
Primary structures of the catalytic subunits from two molecular forms of acetylcholinesterase. A comparison of NH2-terminal and active center sequences.两种分子形式的乙酰胆碱酯酶催化亚基的一级结构。氨基末端和活性中心序列的比较。
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