小鼠免疫球蛋白基因：一种含有前γ2a重链完整编码序列的细菌质粒。
Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-gamma 2a heavy chain.
作者信息
Auffray C, Nageotte R, Chambraud B, Rougeon F
出版信息
Nucleic Acids Res. 1980 Mar 25;8(6):1231-41. doi: 10.1093/nar/8.6.1231.
A DNA sequence complementary to the entire coding part of a mouse gamma 2a immunoglobulin heavy chain mRNA isolated from a myeloma producing a levan binding protein (UPC 10), has been cloned in the PstI site of pBR 322. Transformants containing sequences complementary to purified gamma 2a heavy chain mRNA were selected. One transformant, pG2a-10-21, containing a 1750 nucleotide insert, has been characterized by hybrid-arrested translation and purification of gamma 2a heavy chain mRNA on DNA-DBM cellulose filters. Restriction enzyme analysis and partial sequencing demonstrate that the pG2a-10-21 contains the complete structural sequence for the gamma 2a heavy chain and predicts the sequence of a 18 amino acid hydrophobic amino terminal extra piece segment.
从小鼠骨髓瘤中分离出的、能产生果聚糖结合蛋白（UPC 10）的γ2a免疫球蛋白重链mRNA的整个编码部分的互补DNA序列，已被克隆到pBR 322的PstI位点。筛选出含有与纯化的γ2a重链mRNA互补序列的转化体。一个含有1750个核苷酸插入片段的转化体pG2a-10-21，已通过杂交抑制翻译以及在DNA-二丁基甲酰胺纤维素滤膜上纯化γ2a重链mRNA进行了鉴定。限制性内切酶分析和部分测序表明，pG2a-10-21包含γ2a重链的完整结构序列，并预测了一个18个氨基酸的疏水氨基末端额外片段的序列。
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