Different adenovirus E1A-controlled properties of transformed cells require different levels of E1A expression.

We have transformed primary baby rat kidney cells with a plasmid containing the adenovirus(Ad)12E1 region in which the E1A promoter was replaced by the dexamethasone inducible mouse mammary tumor virus promoter. In the uninduced state the level of E1A expression is less than 10% of that in the induced state. We have investigated the effects of decreasing the levels of E1A on a number of E1A-mediated processes. First, expression of the E1B region is reduced several-fold upon reducing E1A expression. Second, a radical change in cell morphology is observed. Third, despite the decrease in E1A expression, down-regulation of the class-I major histocompatibility complex genes by Ad12E1A is not affected. These results are discussed in terms of different threshold levels of E1A expression required for various E1A-mediated processes.