转座子Tn5插入的物理图谱确定了一个由施氏假单胞菌在氧化亚氮呼吸中发挥作用的基因簇。
Physical mapping of transposon Tn5 insertions defines a gene cluster functional in nitrous oxide respiration by Pseudomonas stutzeri.
作者信息
Viebrock A, Zumft W G
机构信息
Lehrstuhl für Mikrobiologie, Universität Karlsruhe, Federal Republic of Germany.
出版信息
J Bacteriol. 1987 Oct;169(10):4577-80. doi: 10.1128/jb.169.10.4577-4580.1987.
Abstract
By transposon Tn5 mutagenesis, 19 strains of Pseudomonas stutzeri were acquired that had defects in nitrous oxide respiration (Nos- phenotype). A physical map of the mutants showed nearly random Tn5 insertions into genomic DNA within a single region ca. 8 kilobases long. Mutants were characterized immunochemically, enzymatically, and chemically. Several functions related to the synthesis and regulation of nitrous oxide reductase were associated with this DNA region, indicating that in P. stutzeri part of the genetic information necessary to respire nitrous oxide is clustered.
摘要
通过转座子Tn5诱变，获得了19株斯氏假单胞菌菌株，这些菌株在一氧化二氮呼吸方面存在缺陷（Nos-表型）。突变体的物理图谱显示，Tn5几乎随机插入到一个约8千碱基长的单一区域的基因组DNA中。通过免疫化学、酶学和化学方法对突变体进行了表征。与一氧化二氮还原酶的合成和调节相关的几种功能与该DNA区域相关，这表明在斯氏假单胞菌中，一氧化二氮呼吸所需的部分遗传信息是成簇的。
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