Genthner B R, Davis C L, Bryant M P
Appl Environ Microbiol. 1981 Jul;42(1):12-9. doi: 10.1128/aem.42.1.12-19.1981.
Eubacterium limosum was isolated as the most numerous methanol-utilizing bacterium in the rumen fluid of sheep fed a diet in which molasses was a major component (mean most probable number of 6.3 X 10(8) viable cells per ml). It was also isolated from sewage sludge at 9.5 X 10(4) cells per ml. It was not detected in the rumen fluid of a steer on a normal hay-grain diet, although Methanosarcina, as expected, was found at 9.5 X 10(5) cells per ml. The doubling time of E. limosum in basal medium (5% rumen fluid) with methanol as the energy source (37 degree C) was 7 h. Acetate, cysteine, carbon dioxide, and the vitamins biotin, calcium-D-pantothenate, and lipoic acid were required for growth on a chemically defined methanol medium. Acetate, butyrate, and caproate were produced from methanol. Ammonia or each of several amino acids served as the main nitrogen source. Other energy sources included adonitol, arabitol, erythritol, fructose, glucose, isoleucine, lactate, mannitol, ribose, valine, and H2-CO2. The doubling time for growth on H2-CO2 (5% rumen fluid, 37 degree C) was 14 h as compared with 5.2 h for isoleucine and 3.5 h for glucose. The vitamin requirements for growth on H2-CO2 were the same as those for methanol; however, acetate was not required for growth on H2-CO2, although it was necessary for growth on valine, isoleucine, and lactate and was stimulatory to growth on glucose. Acetate and butyrate were formed during growth on H2-CO2, whereas branched-chain fatty acids and ammonia were fermentation products from the amino acids. Heat tolerance was detected, but spores were not observed. The type strain of E. limosum (ATCC 8486) and strain L34, which was isolated from the rumen of a young calf, grew on methanol, H2-CO2, valine, and isoleucine and showed the same requirements for acetate as the freshly isolated strains.
在以糖蜜为主要成分的日粮喂养的绵羊瘤胃液中，多形真杆菌被分离出来，是数量最多的利用甲醇的细菌（平均每毫升最可能数为6.3×10⁸个活细胞）。它也从污水污泥中分离出来，每毫升有9.5×10⁴个细胞。在正常干草-谷物日粮的阉牛瘤胃液中未检测到该菌，不过，正如预期的那样，每毫升发现甲烷八叠球菌9.5×10⁵个细胞。以甲醇为能源（37℃）时，多形真杆菌在基础培养基（5%瘤胃液）中的倍增时间为7小时。在化学限定的甲醇培养基上生长需要乙酸盐、半胱氨酸、二氧化碳以及维生素生物素、泛酸钙和硫辛酸。乙酸盐、丁酸盐和己酸盐由甲醇产生。氨或几种氨基酸中的每一种都可作为主要氮源。其他能源包括卫矛醇、阿拉伯糖醇、赤藓醇、果糖、葡萄糖、异亮氨酸、乳酸、甘露醇、核糖、缬氨酸以及H₂-CO₂。在H₂-CO₂（5%瘤胃液，37℃）上生长的倍增时间为14小时，而异亮氨酸为5.2小时，葡萄糖为3.5小时。在H₂-CO₂上生长的维生素需求与在甲醇上相同；然而，在H₂-CO₂上生长不需要乙酸盐，尽管在缬氨酸、异亮氨酸和乳酸上生长需要乙酸盐，且对葡萄糖生长有刺激作用。在H₂-CO₂上生长期间形成乙酸盐和丁酸盐，而支链脂肪酸和氨是氨基酸的发酵产物。检测到耐热性，但未观察到孢子。多形真杆菌的模式菌株（ATCC 8486）和从一头小牛瘤胃中分离出的L34菌株，能在甲醇、H₂-CO₂、缬氨酸和异亮氨酸上生长，并且对乙酸盐的需求与新分离的菌株相同。
